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Literature summary for 6.3.4.10 extracted from

  • Miyao, H.; Ikeda, Y.; Shiraishi, A.; Kawakami, Y.; Sueda, S.
    Immobilization of immunoglobulin-G-binding domain of Protein A on a gold surface modified with biotin ligase (2015), Anal. Biochem., 484, 113-121 .
    View publication on PubMed

Application

Application Comment Organism
synthesis site-directed antibody immobilization by fusing biotin carboxyl carrier protein BCCP to the IgG-binding domain of Protein A, and the resulting fusion protein is immobilized on the biotin protein ligase-modified gold surface of the sensor chip for quartz crystal microbalance through complexation between BCCP and biotin protein ligase. The layer of the IgG-binding domain is successfully captured the antibody, and the antibody retains high antigen-binding capability Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii F9VNG7 cf. EC 6.3.4.15
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Sulfurisphaera tokodaii DSM 16993 F9VNG7 cf. EC 6.3.4.15
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Synonyms

Synonyms Comment Organism
BPL
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Sulfurisphaera tokodaii