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Literature summary for 6.3.4.10 extracted from

  • Bao, B.; Rodriguez-Melendez, R.; Wijeratne, S.; Zempleni, J.
    Biotin regulates the expression of holocarboxylase synthetase in the miR-539 pathway in HEK-293 cells (2010), J. Nutr., 140, 1546-1551.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
transgenic HEK-293 cells that overexpress HCS fused to green fluorescent protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
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 Homo sapiens
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HEK-293 cell
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 Homo sapiens
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IMR-90 cell
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 Homo sapiens
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JURKAT cell
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 Homo sapiens
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Expression

Organism Comment Expression
Homo sapiens evolutionary conserved binding site for microRNA miR-539 in the 3'-untranslated region of HCS mRNA. miR-539 decreases the expression of HCS at the level of transcription rather than translation. When miR-539 is overexpressed in transgenic cells, the abundance of both HCS and biotinylated histones decreases. The abundance of miR-539 is tissue dependent, decreasing in the order fibroblasts, kidney cells, intestinal cells, lymphoid cells. The abundance of miR-539 is significantly higher at physiological concentrations of biotin than both biotin-deficient and biotin-supplemented media in all cell lines tested down