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Literature summary for 6.3.3.6 extracted from

  • Miller, M.T.; Gerratana, B.; Stapon, A.; Townsend, C.A.; Rosenzweig, A.C.
    Crystal structure of carbapenam synthetase (CarA) (2003), J. Biol. Chem., 278, 40996-41002.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pectobacterium carotovorum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method, the crystal structures of apo-CarA and CarA complexed with the substrate (2S,5S)-5-carboxymethylproline, ATP analog alpha,beta-methyleneadenosine 5'-triphosphate, and a single Mg2+ Pectobacterium carotovorum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity depends on Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55998
-
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data Pectobacterium carotovorum
235000
-
gel filtration Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (2S,5S)-5-carboxymethylproline Pectobacterium carotovorum the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
?

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum Q9XB61
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (2S,5S)-5-carboxymethylproline the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data Pectobacterium carotovorum

Synonyms

Synonyms Comment Organism
CarA
-
Pectobacterium carotovorum