Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pectobacterium carotovorum |
Crystallization (Comment) | Organism |
---|---|
sitting drop method, the crystal structures of apo-CarA and CarA complexed with the substrate (2S,5S)-5-carboxymethylproline, ATP analog alpha,beta-methyleneadenosine 5'-triphosphate, and a single Mg2+ | Pectobacterium carotovorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity depends on | Pectobacterium carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55998 | - |
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data | Pectobacterium carotovorum |
235000 | - |
gel filtration | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2S,5S)-5-carboxymethylproline | Pectobacterium carotovorum | the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis | AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | Q9XB61 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2S,5S)-5-carboxymethylproline | the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis | Pectobacterium carotovorum | AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data | Pectobacterium carotovorum |
Synonyms | Comment | Organism |
---|---|---|
CarA | - |
Pectobacterium carotovorum |