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Literature summary for 6.3.3.6 extracted from

  • Arnett, S.O.; Gerratana, B.; Townsend, C.A.
    Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase (2007), Biochemistry, 46, 9337-9345.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pectobacterium carotovorum

Protein Variants

Protein Variants Comment Organism
K443A at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity Pectobacterium carotovorum
K443M at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity Pectobacterium carotovorum

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type, K443A and K443M mutant enzymes Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (2S,5S)-5-carboxymethylproline a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
 ?

Synonyms

Synonyms Comment Organism
CPS
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pectobacterium carotovorum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 (2S,5S)-5-carboxymethylproline pH 8.0, 25°, wild-type enzyme Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pectobacterium carotovorum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.04
-
 (2S,5S)-5-carboxymethylproline pH 8.0, 25°, wild-type enzyme Pectobacterium carotovorum