Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycoplasma pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution | Mycoplasma pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | Mycoplasma pneumoniae | - |
ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity | Mycoplasma pneumoniae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate | active site and substrate binding site structure | Mycoplasma pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | - |
Mycoplasma pneumoniae | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5,10-Methenyltetrahydrofolate synthetase | - |
Mycoplasma pneumoniae |
Methenyltetrahydrofolate synthetase | - |
Mycoplasma pneumoniae |
MTHFS | - |
Mycoplasma pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycoplasma pneumoniae |