Any feedback?
Literature summary for 6.3.3.2 extracted from
- Cloning, expression, and purification of 5,10-methenyltetrahydrofolate synthetase from Mus musculus (2004), Protein Expr. Purif., 35, 276-283.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as N-terminally His-tagged enzyme, yield of purified recombinant enzyme depends on the growth conditions, M9 media is referred, overview | Mus musculus |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is stabilized by Tween 20 | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
(6S)-5-formyltetrahydrofolate | pH 6.0, 28°C, recombinant enzyme | Mus musculus |  |
| 0.769 | - |
ATP | recombinant enzyme | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mus musculus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | as MgATP2- | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23200 | - |
x * 23200, recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-formyltetrahydrofolate | Mus musculus | the enzyme affects intracellular folate concentrations by accelerating folate degradation | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| ATP + 5-formyltetrahydrofolate | Mus musculus 129/Sv | the enzyme affects intracellular folate concentrations by accelerating folate degradation | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| additional information | Mus musculus | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | ? | - |
? | |
| additional information | Mus musculus 129/Sv | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9D110 | - |
- |
| Mus musculus 129/Sv | Q9D110 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.22 | - |
purified recombinant enzyme | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (6S)-5-formyltetrahydrofolate | - |
Mus musculus | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| ATP + (6S)-5-formyltetrahydrofolate | - |
Mus musculus 129/Sv | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| ATP + 5-formyltetrahydrofolate | ATP in form of MgATP2- | Mus musculus | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydrofolate | ATP in form of MgATP2- | Mus musculus 129/Sv | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydrofolate | the enzyme affects intracellular folate concentrations by accelerating folate degradation | Mus musculus | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| ATP + 5-formyltetrahydrofolate | the enzyme affects intracellular folate concentrations by accelerating folate degradation | Mus musculus 129/Sv | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? | |
| additional information | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | Mus musculus | ? | - |
? | |
| additional information | disruption of folate metabolism is associated with several pathologies and developmental anomalies including cancer and neural tube defects | Mus musculus 129/Sv | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23200, recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,10-Methenyltetrahydrofolate synthetase | - |
Mus musculus |
| MTHFS | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | 28 | assay at | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.3 | - |
(6S)-5-formyltetrahydrofolate | pH 6.0, 28°C, recombinant enzyme | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on, as MgATP2-, binding site motifs | Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
