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Literature summary for 6.3.3.2 extracted from
- Methenyltetrahydrofolate synthetase regulates folate turnover and accumulation (2003), J. Biol. Chem., 278, 29856-29862.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5-formyltetrahydrofolate polyglutamate | induced recombinant enzyme expression in cancer cell cultures, increasing effect with increasing chain length | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in MCF-7 mammary adenocarcinoma cell line and in SH-SY5Y neuroblastoma cell line | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-formyltetrahydrofolate | Mus musculus | - |
ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydrofolate | Homo sapiens | enzyme plays an important role in one-carbon metabolism, overview about folate catabolism | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | low enzyme expression | Mus musculus | - |
| embryo | moderate enzyme expression | Mus musculus | - |
| heart | low enzyme expression | Mus musculus | - |
| kidney | moderate enzyme expression | Mus musculus | - |
| liver | highest enzyme expression | Mus musculus | - |
| lung | low enzyme expression | Mus musculus | - |
| additional information | tissue specific expression | Homo sapiens | - |
| ovary | moderate enzyme expression | Mus musculus | - |
| spleen | low enzyme expression | Mus musculus | - |
| testis | low enzyme expression | Mus musculus | - |
| thymus | moderate enzyme expression | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of recombinantly expressed enzyme in different cell cultures | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-formyltetrahydrofolate | - |
Mus musculus | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydrofolate | enzyme prefers the polyglutamate forms of the substrate compared to the monoglutamate form | Homo sapiens | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
| ATP + 5-formyltetrahydrofolate | enzyme plays an important role in one-carbon metabolism, overview about folate catabolism | Homo sapiens | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methenyltetrahydrofolate synthetase | - |
Mus musculus |
| Methenyltetrahydrofolate synthetase | - |
Homo sapiens |
| MTHFS | - |
Mus musculus |
| MTHFS | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
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Release 2023.1 (January 2023)
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