Literature summary for 6.3.3.2 extracted from

Anguera, M.C.; Suh, J.R.; Ghandour, H.; Nasrallah, I.M.; Selhub, J.; Stover, P.J.
Methenyltetrahydrofolate synthetase regulates folate turnover and accumulation (2003), J. Biol. Chem., 278, 29856-29862.

Search for more literature for 6.3.3.2

Activating Compound

Activating Compound	Comment	Organism	Structure
5-formyltetrahydrofolate polyglutamate	induced recombinant enzyme expression in cancer cell cultures, increasing effect with increasing chain length	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in MCF-7 mammary adenocarcinoma cell line and in SH-SY5Y neuroblastoma cell line	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
cytosol	-	Mus musculus	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 5-formyltetrahydrofolate	Mus musculus	-	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	Homo sapiens	enzyme plays an important role in one-carbon metabolism, overview about folate catabolism	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	low enzyme expression	Mus musculus	-
embryo	moderate enzyme expression	Mus musculus	-
heart	low enzyme expression	Mus musculus	-
kidney	moderate enzyme expression	Mus musculus	-
liver	highest enzyme expression	Mus musculus	-
lung	low enzyme expression	Mus musculus	-
additional information	tissue specific expression	Homo sapiens	-
ovary	moderate enzyme expression	Mus musculus	-
spleen	low enzyme expression	Mus musculus	-
testis	low enzyme expression	Mus musculus	-
thymus	moderate enzyme expression	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity of recombinantly expressed enzyme in different cell cultures	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5-formyltetrahydrofolate	-	Mus musculus	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	?
ATP + 5-formyltetrahydrofolate	enzyme prefers the polyglutamate forms of the substrate compared to the monoglutamate form	Homo sapiens	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	ir
ATP + 5-formyltetrahydrofolate	enzyme plays an important role in one-carbon metabolism, overview about folate catabolism	Homo sapiens	ADP + phosphate + 5,10-methenyltetrahydrofolate	-	ir

Synonyms

Synonyms	Comment	Organism
Methenyltetrahydrofolate synthetase	-	Homo sapiens
Methenyltetrahydrofolate synthetase	-	Mus musculus
MTHFS	-	Homo sapiens
MTHFS	-	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mus musculus

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Release 2026.1 (March 2026)