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Literature summary for 6.3.3.2 extracted from

  • Grimshaw, C.E.; Henderson, G.B.; Soppe, G.G.; Hansen, G.; Mathur, E.J.; Huennekens, F.M.
    Purification and properties of 5,10-methenyltetrahydrofolate synthetase from Lactobacillus casei (1984), J. Biol. Chem., 259, 2728-2733.
    View publication on PubMed
Search for more literature for 6.3.3.2

Inhibitors

Inhibitors Comment Organism Structure
Cl-
-
 Lacticaseibacillus casei
Maleate
-
 Lacticaseibacillus casei
oxalate
-
 Lacticaseibacillus casei
phosphate
-
 Lacticaseibacillus casei
SO42-
-
 Lacticaseibacillus casei
succinate
-
 Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
 ATPgammaS
-
 Lacticaseibacillus casei
0.0006
-
 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei
0.00096
-
 ATP
-
 Lacticaseibacillus casei
0.001
-
 ATP
-
 Lacticaseibacillus casei
0.0013
-
 dATP
-
 Lacticaseibacillus casei
0.0016
-
 CTP
-
 Lacticaseibacillus casei
0.0056
-
 araATP
-
 Lacticaseibacillus casei
0.0064
-
 UTP
-
 Lacticaseibacillus casei
0.0066
-
 dTTP
-
 Lacticaseibacillus casei
0.021
-
 GTP
-
 Lacticaseibacillus casei
0.024
-
 ITP
-
 Lacticaseibacillus casei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no divalent metal ion dependence beyond that required in the MgATP2- substrate complex Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
araATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei araADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
ATPgammaS + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei ADPgammaS + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
CTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei CDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
dATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei dADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
dTTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei dTDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
GTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei GDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ITP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei IDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
UTP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei UDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23000, SDS-PAGE Lacticaseibacillus casei