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Literature summary for 6.3.3.2 extracted from

  • Hopkins, S.; Schirch, V.
    5,10-Methenyltetrahydrofolate synthetase. Purification and properties of the enzyme from rabbit liver (1984), J. Biol. Chem., 259, 5618-5622.
    View publication on PubMed
Search for more literature for 6.3.3.2

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4
-
 Oryctolagus cuniculus
5-methyltetrahydrofolate
-
 Oryctolagus cuniculus
AMP
-
 Oryctolagus cuniculus
MgADP-
-
 Oryctolagus cuniculus
NaCl
-
 Oryctolagus cuniculus
phosphate
-
 Oryctolagus cuniculus
SO42- Na2SO4 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus
0.3
-
 ATP Km-value is constant over the pH-range 5.0-7.5 Oryctolagus cuniculus
0.4
-
 CTP
-
 Oryctolagus cuniculus
0.5
-
 UTP
-
 Oryctolagus cuniculus
0.6
-
 CTP
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ as effective as Mg2+ Oryctolagus cuniculus
Co2+ slightly more effective than Mg2+ Oryctolagus cuniculus
Mg2+ required Oryctolagus cuniculus
Mn2+ as effective as Mg2+ Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 25000, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate substrate binds to the enzyme by a random mechanism, Cleland-type mechanism Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Oryctolagus cuniculus ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
CTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus CDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
CTP + 5-formyltetrahydrofolate CTP in form of MgCTP2- Oryctolagus cuniculus CDP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
GTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus GDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
UTP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus UDP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
UTP + 5-formyltetrahydrofolate UTP in form of MgUTP2- Oryctolagus cuniculus UDP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 below pH 6.5 and above pH 7.5, 4°C, in presence of 2-mercaptoethanol, 24 h, 90% loss of activity Oryctolagus cuniculus
7
-
 4°C, 2-mercaptoethanol, 24 h, 10% loss of activity Oryctolagus cuniculus