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Literature summary for 6.3.3.2 extracted from

  • Huennekens, F.M.; Henderson, G.B.; Vitols, K.S.; Grimshaw, C.E.
    Enzymatic activation of 5-formyltetrahydrofolate via conversion to 5,10-methenyltetrahydrofolate (1984), Adv. Enzyme Regul., 22, 3-13.
    View publication on PubMed
Search for more literature for 6.3.3.2

General Stability

General Stability Organism
enzyme loses activity when subjected to dialysis or concentration by ultrafiltration Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei
0.001
-
 ATP
-
 Lacticaseibacillus casei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lacticaseibacillus casei
Mg2+ required Oryctolagus cuniculus
Mg2+ required Ovis aries

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Lacticaseibacillus casei
25000
-
 gel filtration Oryctolagus cuniculus
26500 29000 gel filtration, HPLC gel filtration, equilibrium ultracentrifugation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Lacticaseibacillus casei
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Ovis aries
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid loss of activity unless kept in the presence of reducing agents, e.g. DTT Lacticaseibacillus casei

Purification (Commentary)

Purification (Comment) Organism
-
 Lacticaseibacillus casei

Reaction

Reaction Comment Organism Reaction ID
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate mechanism is proposed to involve phosphorylation of the formyl group to create an enol phosphate Lacticaseibacillus casei
a

Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell line
-
 Mus musculus
-
L-1210 cell L210 mouse leukemia cells Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-formyltetrahydrofolate
-
 Gallus gallus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Mus musculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Oryctolagus cuniculus ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Ovis aries ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
ATP + 5-formyltetrahydrofolate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23000, SDS-PAGE Lacticaseibacillus casei
monomer 1 * 25000, SDS-PAGE Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Lacticaseibacillus casei
additional information
-
 additional information
-
 Oryctolagus cuniculus