General Stability | Organism |
---|---|
non-ionic detergent, 0.1% v/v Tween 20, and 10% v/v glycerol stabilize the enzyme which is extremely labile at 4 C | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-N10-methylpteroyl pentaglutamate | i.e. methotrexate pentaglutamate | Homo sapiens | |
dihydropteroyl pentaglutamate | potent | Homo sapiens | |
dihydropteroyl-L-Glu | weak | Homo sapiens | |
additional information | no inhibition by methotrexate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
(6S)-5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | |
0.002 | - |
(6S)-5-formyltetrahydrofolate | - |
Homo sapiens | |
0.004 | - |
(6R,S)-5-formyltetrahydrofolate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Homo sapiens |
27000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate | sequential mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, pH 7.0, 0.1% v/v Tween 20, 10% v/v glycerol, stable for several months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6R,S)-5-formyltetrahydrofolate | - |
Homo sapiens | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
ATP + (6S)-5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | ? | - |
? | |
ATP + 5-formyltetrahydrofolate | no reverse reaction detected | Homo sapiens | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | (6R,S)-5-formyltetrahydrofolate, (6S)-5-formyltetrahydrofolate | Homo sapiens | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
extremely labile at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
cytosolic enzyme | Homo sapiens |