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Literature summary for 6.3.3.2 extracted from

  • Bertrand, R.; MacKenzie, R.E.; Jolivot, J.
    Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987), Biochim. Biophys. Acta, 911, 154-161.
    View publication on PubMed

General Stability

General Stability Organism
non-ionic detergent, 0.1% v/v Tween 20, and 10% v/v glycerol stabilize the enzyme which is extremely labile at 4 C Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-amino-N10-methylpteroyl pentaglutamate i.e. methotrexate pentaglutamate Homo sapiens
dihydropteroyl pentaglutamate potent Homo sapiens
dihydropteroyl-L-Glu weak Homo sapiens
additional information no inhibition by methotrexate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
(6S)-5-formyltetrahydropteroyl-pentaglutamate
-
Homo sapiens
0.002
-
(6S)-5-formyltetrahydrofolate
-
Homo sapiens
0.004
-
(6R,S)-5-formyltetrahydrofolate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
gel filtration Homo sapiens
27000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate sequential mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, pH 7.0, 0.1% v/v Tween 20, 10% v/v glycerol, stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6R,S)-5-formyltetrahydrofolate
-
Homo sapiens ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + (6S)-5-formyltetrahydropteroyl-pentaglutamate
-
Homo sapiens ?
-
?
ATP + 5-formyltetrahydrofolate no reverse reaction detected Homo sapiens ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate (6R,S)-5-formyltetrahydrofolate, (6S)-5-formyltetrahydrofolate Homo sapiens ADP + phosphate + 5,10-methylenetetrahydrofolate
-
?
ATP + 5-formyltetrahydropteroyl-pentaglutamate
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
extremely labile at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
cytosolic enzyme Homo sapiens