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Literature summary for 6.3.3.2 extracted from
- Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987), Biochim. Biophys. Acta, 911, 154-161.
General Stability
| General Stability | Organism |
|---|---|
| non-ionic detergent, 0.1% v/v Tween 20, and 10% v/v glycerol stabilize the enzyme which is extremely labile at 4 C | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-amino-N10-methylpteroyl pentaglutamate | i.e. methotrexate pentaglutamate | Homo sapiens |  |
| dihydropteroyl pentaglutamate | potent | Homo sapiens | |
| dihydropteroyl-L-Glu | weak | Homo sapiens | |
| additional information | no inhibition by methotrexate | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
(6S)-5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | |
| 0.002 | - |
(6S)-5-formyltetrahydrofolate | - |
Homo sapiens | |
| 0.004 | - |
(6R,S)-5-formyltetrahydrofolate | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Homo sapiens |
| 27000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate | sequential mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, pH 7.0, 0.1% v/v Tween 20, 10% v/v glycerol, stable for several months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (6R,S)-5-formyltetrahydrofolate | - |
Homo sapiens | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
| ATP + (6S)-5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | ? | - |
? | |
| ATP + 5-formyltetrahydrofolate | no reverse reaction detected | Homo sapiens | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydrofolate | (6R,S)-5-formyltetrahydrofolate, (6S)-5-formyltetrahydrofolate | Homo sapiens | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
? | |
| ATP + 5-formyltetrahydropteroyl-pentaglutamate | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
extremely labile at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
cytosolic enzyme | Homo sapiens |
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