Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a histidine-tagged enzyme | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | double mutant R35C/E57G obtained by site-directed mutagenesis | Salmonella enterica |
additional information | triple mutant R35C/E57G/R198W obtained by site-directed mutagenesis | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
2-(formamido)-N1-(5-phosphoribosyl)acetamidine | 37°C, pH 7.7, wild type enzyme | Salmonella enterica | |
0.066 | - |
2-(formamido)-N1-(5-phosphoribosyl)acetamidine | 37°C, pH 7.7, R35C/E57G mutant enzyme | Salmonella enterica | |
0.126 | - |
ATP | 37°C, pH 7.7, wild type enzyme | Salmonella enterica | |
1.68 | - |
ATP | 37°C, pH 7.7, R35C/E57G mutant enzyme | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | P74883 | serovar typhimurium | - |
Purification (Comment) | Organism |
---|---|
purification of a histidine-tagged enzyme by nickel-affinity column chromatography | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine | - |
Salmonella enterica | ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminoimidazole ribonucleotide synthetase | - |
Salmonella enterica |