Literature summary for 6.3.3.1 extracted from

Mueller, E.J.; Oh, S.; Kavalerchik, E.; Kappock, T.J.; Meyer, E.; Li, C.; Ealick, S.E.; Stubbe, J.
Investigation of the ATP Binding Site of Escherichia coli Aminoimidazole Ribonucleotide Synthetase Using Affinity Labeling and Site-Directed Mutagenesis (1999), Biochemistry, 38, 9831-9839.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of recombinant enzymes in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K27L	obtained by site-directed mutagenesis and expression in Escherichia coli	Escherichia coli
K27Q	obtained by site-directed mutagenesis and expression in Escherichia coli	Escherichia coli
K27R	obtained by site-directed mutagenesis and expression in Escherichia coli	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
fluorosulfonylbenzoyl adenosine	FSBA, time-dependent inactivation by covalent binding to Lys27	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	ATP	pH 7.7, K27R mutant	Escherichia coli
0.046	-	ATP	pH 7.7, K27L mutant	Escherichia coli
0.058	-	ATP	pH 7.7, K27Q mutant	Escherichia coli
0.073	-	ATP	pH 7.7, wild type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P08178	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of wild type and recombinant enzymes using C8-linked ATP affinity column	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine	-	Escherichia coli	ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole	-	?

Synonyms

Synonyms	Comment	Organism
aminoimidazole ribonucleotide synthetase	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.025	-	fluorosulfonylbenzoyl adenosine	15°C, pH 7.7	Escherichia coli

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Release 2023.1 (January 2023)