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Literature summary for 6.3.2.B4 extracted from

  • Lalle, M.; Camerini, S.; Cecchetti, S.; Fantauzzi, C.; Crescenzi, M.; Pozio, E.
    Giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosine ligase-like member and deglycylated by two metallocarboxypeptidases (2011), J. Biol. Chem., 286, 4471-4484.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.B4

Cloned(Commentary)

Cloned (Comment) Organism
gene gtll3, phylogenetic analysis, RNA reverse transcription and real-time quantitative PCR expression analysis, expression of the N-terminally FLAG-tagged enzyme, under the constitutive promoter of the Gardia duodenalis alpha-tubulin gene, in Gardia duodenalis transgenic trophozoites, subcloning in Escherichia coli Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 14-3-3 protein + glycine Giardia intestinalis
-
 glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + 14-3-3 protein + glycine Giardia intestinalis WBC6
-
 glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + alpha-tubulin + glycine Giardia intestinalis
-
 glycyl-alpha-tubulin + ADP + phosphate
-
 ?
ATP + alpha-tubulin + glycine Giardia intestinalis WBC6
-
 glycyl-alpha-tubulin + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Giardia intestinalis WBC6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally FLAG-tagged enzyme from Gardia duodenalis trophozoites by FLAG affinity chromatography Giardia intestinalis

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Giardia intestinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 14-3-3 protein + glycine
-
 Giardia intestinalis glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + 14-3-3 protein + glycine no activity with 14-3-3 proteinmutant E246A Giardia intestinalis glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + 14-3-3 protein + glycine
-
 Giardia intestinalis WBC6 glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + 14-3-3 protein + glycine no activity with 14-3-3 proteinmutant E246A Giardia intestinalis WBC6 glycyl-14-3-3 protein + ADP + phosphate
-
 ?
ATP + alpha-tubulin + glycine
-
 Giardia intestinalis glycyl-alpha-tubulin + ADP + phosphate
-
 ?
ATP + alpha-tubulin + glycine
-
 Giardia intestinalis WBC6 glycyl-alpha-tubulin + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000-520000, recombinant FLAG-tagged enzyme, SDS-PAGE Giardia intestinalis

Synonyms

Synonyms Comment Organism
giardial TTLL3 protein
-
 Giardia intestinalis
gTTLL3
-
 Giardia intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Giardia intestinalis

General Information

General Information Comment Organism
evolution the giardial TTLL3 protein, a member of the TTLL family Giardia intestinalis
additional information production of FLAG-gTTLL3 protein in trophozoites does not affect the deglycylation of 14-3-3 protein during encystation, 14-3-3 protein localization, or the encystation timing. The E246A mutant of 14-3-3 protein, which cannot be glycylated, localizes to the nuclei already at the trophozoite stage, resulting in a faster differentiation of trophozoites into cysts Giardia intestinalis
physiological function Giardial gTTLL3 is responsible for the 14-3-3 protein and tubulin polyglycylation in vivo Giardia intestinalis