BRENDA - Enzyme Database
show all sequences of 6.3.2.B24

TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites

Ikegami, K.; Mukai, M.; Tsuchida, J.; Heier, R.L.; Macgregor, G.R.; Setou, M.; J. Biol. Chem. 281, 30707-30716 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
glutathione S-transferase-fused mTTLL7 is synthesized in Escherichia coli Rossetta strain (DE3)
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
Mus musculus
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
A4Q9F0
-
-
Purification (Commentary)
Commentary
Organism
-
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
TTLL7 is distributed in a somatodendritic compartment in the mouse brain
Mus musculus
-
nervous system
-
Mus musculus
-
PC-12 cell
-
Mus musculus
-
superior cervical ganglion
consistent with having a role in the growth of MAP2-positive neurites, TTLL7 accumulates within a MAP2-enriched somatodendritic portion of superior cervical ganglion, as does polyglutamylated beta-tubulin
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
751019
Mus musculus
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
-
?
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
the enzyyme catalyzes tubulin polyglutamylation with high preference to beta-tubulin in vitro. When expressed in HEK293T cells, TTLL7 demonstrates specificity for beta-tubulin and not for alpha-tubulin or nucleosome assembly protein 1
751019
Mus musculus
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
glutathione S-transferase-fused mTTLL7 is synthesized in Escherichia coli Rossetta strain (DE3)
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
Mus musculus
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
TTLL7 is distributed in a somatodendritic compartment in the mouse brain
Mus musculus
-
nervous system
-
Mus musculus
-
PC-12 cell
-
Mus musculus
-
superior cervical ganglion
consistent with having a role in the growth of MAP2-positive neurites, TTLL7 accumulates within a MAP2-enriched somatodendritic portion of superior cervical ganglion, as does polyglutamylated beta-tubulin
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
751019
Mus musculus
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
-
?
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate
the enzyyme catalyzes tubulin polyglutamylation with high preference to beta-tubulin in vitro. When expressed in HEK293T cells, TTLL7 demonstrates specificity for beta-tubulin and not for alpha-tubulin or nucleosome assembly protein 1
751019
Mus musculus
[beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
-
-
?
Expression
Organism
Commentary
Expression
Mus musculus
following stimulation of PC12 cells with nerve growth factor to differentiate, the level of TTLL7 increases concomitantly with polyglutamylation of beta-tubulin
up
General Information
General Information
Commentary
Organism
malfunction
knockdown of TTLL7 in a primary culture of superior cervical ganglion neurons causes a loss of polyglutamylated beta-tubulin. Short interference RNA-mediated knockdown of TTLL7 represses nerve growth factor-stimulated MAP (microtubule-associated protein) 2-positive neurite growth in PC12 cells
Mus musculus
physiological function
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
knockdown of TTLL7 in a primary culture of superior cervical ganglion neurons causes a loss of polyglutamylated beta-tubulin. Short interference RNA-mediated knockdown of TTLL7 represses nerve growth factor-stimulated MAP (microtubule-associated protein) 2-positive neurite growth in PC12 cells
Mus musculus
physiological function
the enzyme is required for the growth of MAP2-positive neurites in PC12 cells
Mus musculus
Expression (protein specific)
Organism
Commentary
Expression
Mus musculus
following stimulation of PC12 cells with nerve growth factor to differentiate, the level of TTLL7 increases concomitantly with polyglutamylation of beta-tubulin
up
Other publictions for EC 6.3.2.B24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744023
Schumacher
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749888
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Mus musculus
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2009
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5
12
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1
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751550
van Dijk
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Mol. Cell
26
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2007
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1
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751019
Ikegami
TTLL7 is a mammalian beta-tub ...
Mus musculus
J. Biol. Chem.
281
30707-30716
2006
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