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Literature summary for 6.3.2.B2 extracted from

  • Das, D.; Herve, M.; Feuerhelm, J.; Farr, C.; Chiu, H.; Elsliger, M.; Knuth, M.; Klock, H.; Miller, M.; Godzik, A.; Lesley, S.; Deacon, A.; Mengin-Lecreulx, D.; Wilson, I.
    Structure and function of the first full-length murein peptide ligase (Mpl) cell wall recycling protein (2011), PLoS ONE, 6, e17624.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.B2

Cloned(Commentary)

Cloned (Comment) Organism
gene mpl, chromosomal mpl gene, DNA and mino acid sequence determination and analysis, expression of His-tagged enzyme in an Escherichia coli chromosomal mpl gene-deficient mutant strain MLD2502 Psychrobacter arcticus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution Psychrobacter arcticus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Psychrobacter arcticus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Psychrobacter arcticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE Psychrobacter arcticus
57187
-
 x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE Psychrobacter arcticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc Psychrobacter arcticus
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc Psychrobacter arcticus 273-4
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
-
 ?

Organism

Organism UniProt Comment Textmining
Psychrobacter arcticus Q4FVQ2 gene mpl
-
Psychrobacter arcticus 273-4 Q4FVQ2 gene mpl
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain MLD2502 by nickel and cobalt affinity chromatography Psychrobacter arcticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
ATP + L-alanine + UDP-MurNAc
-
 Psychrobacter arcticus 273-4 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
-
 ?
ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
-
 Psychrobacter arcticus 273-4 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
-
 Psychrobacter arcticus 273-4 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-L-lysyl-D-alanine
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
-
 Psychrobacter arcticus 273-4 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
-
 ?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine + UDP-MurNAc
-
 Psychrobacter arcticus ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
-
 ?
additional information substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala Psychrobacter arcticus ?
-
 ?
additional information substrate specificity of Mpl, overview. No activity with L-Ala-c-D-Glu-L-Lys-D-Ala-D-Ala Psychrobacter arcticus 273-4 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE Psychrobacter arcticus
More PaMpl can be divided into 3 distinct domains: the N-terminal UDPMurNAc-binding domain (residues 1-102), the middle ATP-binding domain (residues 103-357) and the C-terminal tripeptide-binding domain (residues 358-505) Psychrobacter arcticus

Synonyms

Synonyms Comment Organism
Mpl
-
 Psychrobacter arcticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
0.0042
-
 L-alanyl-gamma-D-glutamyl-L-lysine pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
0.0117
-
 L-alanine pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
0.023
-
 L-alanyl-gamma-D-glutamate pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
0.088
-
 L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
0.098
-
 L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus
4.33
-
 L-alanyl-gamma-D-glutamyl-meso-diaminopimelate pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme Psychrobacter arcticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 assay at Psychrobacter arcticus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Psychrobacter arcticus

General Information

General Information Comment Organism
evolution Mpl and MurC-F enzymes have three domains that are classified in Pfam families, PF01225 Mur ligase catalytic domain, PF08245 Mur ligase middle domain, and PF02875 Mur ligase glutamate binding/C-terminal domain, structure comparisons, overview Psychrobacter arcticus
additional information unique sequence-structure relationships of Mpl proteins, overview, Residues Arg357, Arg358, Phe374, Ala375, His376, His377, Glu402, Pro403, Arg404, Ser405, Asn406, Thr407, Ser483, Asn484 and Gly485 may be important in binding tri, tetra, and pentapeptide substrates Psychrobacter arcticus
physiological function in Gram-negative bacteria, 30-60% of the bacterial cell wall is recycled during each generation. Part of this recycling process involves the murein peptide ligase, which attaches the breakdown product, the tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to UDP-MurNAc Psychrobacter arcticus