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Literature summary for 6.3.2.B11 extracted from

  • Collard, F.; Stroobant, V.; Lamosa, P.; Kapanda, C.N.; Lambert, D.M.; Muccioli, G.G.; Poupaert, J.H.; Opperdoes, F.; van Schaftingen, E.
    Molecular identification of N-acetylaspartylglutamate synthase and beta-citrylglutamate synthase (2010), J. Biol. Chem., 285, 29826-29833.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.B11

Cloned(Commentary)

Cloned (Comment) Organism
produced it in Escherichia coli either unmodified or as a fusion protein with a poly-His tag at the C terminus. Extracts of cells expressing these recombinant proteins display N-acetylaspartylglutamate synthase activity Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 ATP pH 8.0, 30°C Mus musculus
0.73
-
 L-glutamate pH 8.0, 30°C Mus musculus
1.24
-
 citrate pH 8.0, 30°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + citrate + L-glutamate Mus musculus the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis ADP + phosphate + N-citryl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q80WS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
central nervous system
-
 Mus musculus
-
placenta
-
 Mus musculus
-
testis
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + citrate + L-glutamate the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis Mus musculus ADP + phosphate + N-citryl-L-glutamate
-
 ?
ATP + citrate + L-glutamate Vmax/Km for the synthesis of beta-citrylglutamate is 4fold higher than the Vmax/Km value for the synthesis of N-acetylaspartyl-glutamate Mus musculus ADP + phosphate + N-citryl-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetylaspartylglutamate synthase ambiguous Mus musculus
NAAG synthetase B
-
 Mus musculus
NAAGS B
-
 Mus musculus
RIMKLB
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information from the Vmax, kcat values of 3 and 1.5/s are calculated for the beta-citrylglutamate synthase activity and and N-acetylaspartyl-glutamate synthase activity Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 synthesis of N-citryl-L-glutamate Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 the rates for beta-citrylglutamate synthesis and N-acetylaspartyl-glutamate synthesis are similar between pH 7 and 8, but the beta-citrylglutamate synthetase is severalfold higher than the N-acetylaspartyl-glutamate synthetase activity at more alkaline pH Mus musculus
7.5 9 pH 7.5: about 40% of maximal activity, pH 9.0: about 95% of maximal activity, synthesis of N-citryl-L-glutamate Mus musculus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the mammalian brain Mus musculus