Application | Comment | Organism |
---|---|---|
drug development | MurD is a target for small molecule chemotherapeutics | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CPAHWPHPC | i.e. peptide MD-C7C_1, inhibits MurD | Escherichia coli | |
CSAWSNKFC | i.e. peptide MD-C7C_2, inhibits MurD | Escherichia coli | |
HSSWYIQHFPPL | i.e. peptide MD-12, inhibits MurD | Escherichia coli | |
additional information | design of peptidomimetic lead compounds with the ultimate objective of small molecule chemotherapeutic development, affinity selection by immobilized MurD with addition of ATP in an attempt to achieve conformationally homogenous population of target enzymes, overview. No inhibition by peptide MD-C7C_3, i.e. CQSSPHMSC | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | Escherichia coli | MurD is essential for cytoplasmic bacterial cell wall biosynthesis | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene murD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | MurD is essential for cytoplasmic bacterial cell wall biosynthesis | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | all Mur ligases are topologically similar to each other, extremely flexible, and built of three domains connected by two hinges. Each domain binds one of the three substrates, i.e. ATP, growing nucleotide, or the appropriate amino acid | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
MurD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.14 | - |
pH 8.0, 37°C | Escherichia coli | HSSWYIQHFPPL | |
0.62 | - |
pH 8.0, 37°C | Escherichia coli | CSAWSNKFC | |
1.5 | - |
pH 8.0, 37°C | Escherichia coli | CPAHWPHPC |