Crystallization (Comment) | Organism |
---|---|
vapour-diffusion method and hanging-drop system, high resolution crystal structures of MurD in complexes with two novel inhibitors designed to mimic the transition state of the reaction, N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid or N-(6-butoxy-naphthalene-2-sulfonyl)-Lglutamic acid | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid | competitive versus D-Glu, non-competitive versus ATP and UDP-N-acetylmuramoyl-L-Ala | Escherichia coli | |
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid | competitive versus D-Glu, non-competitive versus UDP-N-acetylmuramoyl-L-Ala | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
UDP-N-acetylmuramoyl-L-Ala | - |
Escherichia coli | |
0.09 | - |
D-Glu | - |
Escherichia coli | |
0.095 | - |
ATP | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P14900 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu | highly stereospecific for D-Glu | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurD ligase | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid | versus D-Glu | Escherichia coli | |
0.71 | - |
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid | versus UDP-N-acetylmuramoyl-L-Ala | Escherichia coli | |
0.87 | - |
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid | versus D-Glu | Escherichia coli | |
1.66 | - |
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid | versus ATP | Escherichia coli | |
2.13 | - |
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid | versus UDP-N-acetylmuramoyl-L-Ala | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.28 | - |
- |
Escherichia coli | N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid | |
0.71 | - |
- |
Escherichia coli | N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid |