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Literature summary for 6.3.2.9 extracted from

  • El-Sherbeini, M.; Geissler, W.M.; Pittman, J.; Yuan, X.; Wong, K.K.; Pompliano, D.L.
    Cloning and expression of Staphylococcus aureus and Streptococcus pyogenes murD genes encoding uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligases (1998), Gene, 210, 117-125.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tagged enzyme in Escherichia coli BL21 DE3/pLysS Staphylococcus pyogenes
expressed as His-tagged enzyme in Escherichia coli BL21 DE3/pLysS Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus P0A091 clinical isolates of strain R27
-
Staphylococcus pyogenes
-
-
-
Staphylococcus pyogenes MB4439
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes chromatography by a Talon resin Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
25°C Staphylococcus aureus