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Literature summary for 6.3.2.8 extracted from

  • Emanuele, J.J.; Jin, H.; Jacobson, B.L.; Chang, C.Y.; Einspahr, H.M.; Villafranca, J.J.
    Kinetic and crystallographic studies of Escherichia coli UDP-N-acetylmuramate:L-alanine ligase (1996), Protein Sci., 5, 2566-2574.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.8

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 UDP-N-acetylmuramate
-
 Escherichia coli
0.046
-
 beta-Ala
-
 Escherichia coli
0.048
-
 L-Ala
-
 Escherichia coli
0.13
-
 ATP
-
 Escherichia coli
0.14
-
 ATP beta-chloro-L-Ala Escherichia coli
0.19
-
 beta-cyano-L-Ala
-
 Escherichia coli
0.2
-
 L-Ala 2-amino-tert-butylpropionate Escherichia coli
0.22
-
 UDP-N-acetyl-alpha-D-muramate
-
 Escherichia coli
0.36
-
 benzyl L-alaninate
-
 Escherichia coli
0.44
-
 L-Vinylglycine
-
 Escherichia coli
0.5
-
 methyl L-alaninate
-
 Escherichia coli
0.59
-
 ethyl L-alaninate
-
 Escherichia coli
1.1
-
 DL-propargylglycine
-
 Escherichia coli
1.2
-
 L-Ser
-
 Escherichia coli
3.8
-
 L-Cys
-
 Escherichia coli
4.3
-
 2-amino-n-butyric acid
-
 Escherichia coli
7.9
-
 2-aminopropionamide
-
 Escherichia coli
10
-
 Gly
-
 Escherichia coli
16
-
 2-amino-N-hydroxymethyl propionamide
-
 Escherichia coli
25
-
 L-Thr
-
 Escherichia coli
40
-
 L-Ile
-
 Escherichia coli
62
-
 N-(2-aminopropionyl)piperidine
-
 Escherichia coli
70
-
 L-Val
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ support activity Escherichia coli
Mg2+ support activity Escherichia coli
Mn2+ supports activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UDP-N-acetylmuramate + L-Ala Escherichia coli adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
-
 Escherichia coli ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-benzylpropionate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-benzylpropionate
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-ethylpropionate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-ethylpropionate
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-methylpropionate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-methylpropionate
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-N-hydroxymethyl propionamide
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-hydroxymethylpropionamide
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-propionamide
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-aminopropionamide
-
 ?
ATP + UDP-N-acetylmuramate + 2-amino-tert-butylpropionate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-tert-butylpropionate
-
 ?
ATP + UDP-N-acetylmuramate + benzyl L-alaninate
-
 Escherichia coli ADP + phosphate + benzyl UDP-N-acetylmuramoyl-L-alanine
-
 ?
ATP + UDP-N-acetylmuramate + beta-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-beta-Ala
-
 ?
ATP + UDP-N-acetylmuramate + beta-chloro-L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-beta-chloro-L-Ala
-
 ?
ATP + UDP-N-acetylmuramate + beta-cyano-L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-beta-cyano-L-Ala
-
 ?
ATP + UDP-N-acetylmuramate + DL-propargylglycine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-propargylglycine
-
 ?
ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
ATP + UDP-N-acetylmuramate + ethyl L-alaninate
-
 Escherichia coli ADP + phosphate + ethyl UDP-N-acetylmuramoyl-L-alaninate
-
 ?
ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis Escherichia coli ?
-
 ?
ATP + UDP-N-acetylmuramate + L-Cys
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Cys
-
 ?
ATP + UDP-N-acetylmuramate + L-Ile
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ile
-
 ?
ATP + UDP-N-acetylmuramate + L-serine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?
ATP + UDP-N-acetylmuramate + L-Thr
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Thr
-
 ?
ATP + UDP-N-acetylmuramate + L-Val
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Val
-
 ?
ATP + UDP-N-acetylmuramate + L-vinylglycine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-vinylglycine
-
 ?
ATP + UDP-N-acetylmuramate + methyl L-alaninate
-
 Escherichia coli ADP + phosphate + methyl UDP-N-acetylmuramoyl-L-alaninate
-
 ?
ATP + UDP-N-acetylmuramate + N-(2-aminopropionyl)piperidine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-N-(2-aminopropionyl)piperidine
-
 ?
additional information the enzyme hydrolyzes ATP in absence of L-Ala. This L-Ala independent activity is dependent upon the concentration of both ATP and UDP-N-acetylmuramate Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
2.5
-
 beta-Alanine
-
 Escherichia coli
5.17
-
 L-Ile
-
 Escherichia coli
6.17
-
 N-(2-aminopropionyl)piperidine
-
 Escherichia coli
11.7
-
 2-amino-N-hydroxymethylpropionamide
-
 Escherichia coli
13.5
-
 2-amino-benzylpropionate
-
 Escherichia coli
15.2
-
 2-amino-ethylpropionate
-
 Escherichia coli
15.3
-
 L-Vinylglycine
-
 Escherichia coli
16.3
-
 L-Ala
-
 Escherichia coli
18.3
-
 2-amino-propionamide
-
 Escherichia coli
20
-
 L-Cys
-
 Escherichia coli
20
-
 DL-propargylglycine
-
 Escherichia coli
20
-
 beta-chloro-Ala
-
 Escherichia coli
21.7
-
 Gly
-
 Escherichia coli
21.7
-
 L-Val
-
 Escherichia coli
21.7
-
 2-amino-tert-butylpropionate
-
 Escherichia coli
23.3
-
 L-Thr
-
 Escherichia coli
25
-
 L-Ser
-
 Escherichia coli
25
-
 2-amino-n-butyric acid
-
 Escherichia coli
26.7
-
 beta-cyano-L-Ala
-
 Escherichia coli
28.3
-
 2-amino-methylpropionate
-
 Escherichia coli