Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged Ttll1p mainly in the basal bodies and of GFP-tagged Ttll9p mainly in the cilia of CU428 and B2086 cells | Tetrahymena thermophila |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of the TTLL1 and TTLL9 genes decrease the rates of cell multiplication and phagocytosis. Cells lacking both genes have fewer cortical microtubules and show defects in the maturation of basal bodies, in the TTLL1 and TTLL9 double knockout, a decrease in the levels of glutamylation on alpha-tubulin occurs, phenotype, overview | Tetrahymena thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cilium | Ttll9p | Tetrahymena thermophila | 5929 | - |
flagellar basal body | Ttll1p | Tetrahymena thermophila | - |
- |
microtubule | - |
Tetrahymena thermophila | 5874 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Tetrahymena thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-tubulin + L-glutamate | Tetrahymena thermophila | glutamylation on alpha-tubulin is not essential but is required for efficiency of assembly and function of a subset of microtubule-based organelles, the spatial restriction of modifying enzymes appears to be a major mechanism that drives differential glutamylation at the subcellular level | L-glutamyl-alpha-tubulin + ADP + phosphate | - |
? | |
additional information | Tetrahymena thermophila | Ttll1p and Ttll9p are tubulin tyrosine ligase domain proteins, that act as alpha-tubulin-preferring glutamyl ligase enzymes, TTLL1- and TLLL9-type enzymes are highly conserved but are absent from higher plants and fungi, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetrahymena thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of recombinant GFP-tagged Ttll1p and GFP-tagged Ttll9p | Tetrahymena thermophila |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [tubulin]-L-glutamate + L-glutamate = [tubulin]-(gamma-L-glutamyl)-L-glutamate + ADP + phosphate | the enzymatic mechanism of microtubule glutamylation involves two distinct steps: initiation and elongation | Tetrahymena thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-tubulin + L-glutamate | glutamylation on alpha-tubulin is not essential but is required for efficiency of assembly and function of a subset of microtubule-based organelles, the spatial restriction of modifying enzymes appears to be a major mechanism that drives differential glutamylation at the subcellular level | Tetrahymena thermophila | L-glutamyl-alpha-tubulin + ADP + phosphate | - |
? | |
ATP + alpha-tubulin + L-glutamate | in an in vitro microtubule glutamylation assay, Ttll1p shows a chain-initiating activity while Ttll9p has primarily a chain-elongating activity. MALDI-TOF mass spectrometry analysis of glutamylated forms of ciliary alpha-tubulin | Tetrahymena thermophila | L-glutamyl-alpha-tubulin + ADP + phosphate | - |
? | |
additional information | Ttll1p and Ttll9p are tubulin tyrosine ligase domain proteins, that act as alpha-tubulin-preferring glutamyl ligase enzymes, TTLL1- and TLLL9-type enzymes are highly conserved but are absent from higher plants and fungi, overview | Tetrahymena thermophila | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-tubulin-preferring glutamyl ligase | - |
Tetrahymena thermophila |
More | tubulin glutamylases belong to the family of TTL domain-carrying proteins named TTLL proteins | Tetrahymena thermophila |
TTL domain protein | - |
Tetrahymena thermophila |
Ttll1p | - |
Tetrahymena thermophila |
Ttll9p | - |
Tetrahymena thermophila |
tubulin glutamylase | - |
Tetrahymena thermophila |
tubulin tyrosine ligase domain protein | - |
Tetrahymena thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tetrahymena thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Tetrahymena thermophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Tetrahymena thermophila |