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Literature summary for 6.3.2.60 extracted from

  • Ouchi, T.; Tomita, T.; Horie, A.; Yoshida, A.; Takahashi, K.; Nishida, H.; Lassak, K.; Taka, H.; Mineki, R.; Fujimura, T.; Kosono, S.; Nishiyama, C.; Masui, R.; Kuramitsu, S.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus (2013), Nat. Chem. Biol., 9, 277-283 .
    View publication on PubMed
Search for more literature for 6.3.2.60

Cloned(Commentary)

Cloned (Comment) Organism
gene argX, sequence comparisons and phylogenetic analysis Sulfolobus acidocaldarius

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in complex with ADP and with substrate StLysW, X-ray diffraction structure determination and analysis at 1.8 A resolution Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sulfolobus acidocaldarius
additional information LysW contains an N-terminally bound Zn2+ Sulfolobus acidocaldarius
sulfate enzyme bound, the sulfate ion is replaced with the phosphate moiety of acylphosphate intermediate of StLysW in the structure model Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius DSM 639
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius ATCC 33909
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius NBRC 15157
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius NCIMB 11770
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate Sulfolobus acidocaldarius JCM 8929
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J8E7
-
-
Sulfolobus acidocaldarius ATCC 33909 Q4J8E7
-
-
Sulfolobus acidocaldarius DSM 639 Q4J8E7
-
-
Sulfolobus acidocaldarius JCM 8929 Q4J8E7
-
-
Sulfolobus acidocaldarius NBRC 15157 Q4J8E7
-
-
Sulfolobus acidocaldarius NCIMB 11770 Q4J8E7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius ATCC 33909 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius NBRC 15157 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius NCIMB 11770 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + L-glutamate + [LysW]-C-terminal-L-glutamate
-
 Sulfolobus acidocaldarius JCM 8929 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
ArgX
-
 Sulfolobus acidocaldarius
More cf. EC 6.3.2.43 Sulfolobus acidocaldarius
StArgX
-
 Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sulfolobus acidocaldarius

General Information

General Information Comment Organism
evolution gene duplication events at different stages of evolution led to ArgX and LysX (EC 6.3.2.43) Sulfolobus acidocaldarius
metabolism lysine and arginine biosyntheses are mediated by a common carrier protein in Sulfolobus acidocaldarius. In this archaeon, after LysW modification, AAA and glutamate are converted to lysine and ornithine, respectively, by a single set of enzymes with dual functions Sulfolobus acidocaldarius
additional information StArgX/ADP structrue analysis, and structure of substrate StLysW and recognition by enzyme StArgX, structural model of the phosphoryl intermediate in the StArgX reaction, overview. The alpha-amino group of the substrate glutmate molecule is to be ligated with the gamma-carboxyl group of Glu56 residue of StLysW. Interaction analysis of ArgX and LysW Sulfolobus acidocaldarius
physiological function the hyperthermophilic archaeon Sulfolobus acidocaldarius not only biosynthesizes lysine through LysW-mediated protection of alpha-aminoadipate (AAA), but also uses the amino-group carrier protein LysW to protect the amino group of glutamate in arginine biosynthesis, cf. EC 6.3.2.43. ArgX is the enzyme responsible for modification and protection of the amino moiety of glutamate with LysW Sulfolobus acidocaldarius