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Literature summary for 6.3.2.6 extracted from

  • Tuntland, M.L.; Wolf, N.M.; Fung, L.W.
    Differences in the purification and solution properties of PurC gene products from Streptococcus pneumoniae and Bacillus anthracis (2015), Protein Expr. Purif., 114, 143-148 .
    View publication on PubMed

Application

Application Comment Organism
drug development 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen Streptococcus pneumoniae
drug development 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is an ideal target for the discovery of antimicrobials against the pathogen Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Streptococcus pneumoniae
gene purC, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pneumoniae
Mg2+ required Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
recombinant enzyme, gel filtration Bacillus anthracis
56000
-
recombinant enzyme, gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae
-
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Bacillus anthracis
-
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae ATCC BAA-334 / TIGR4
-
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81ZH5
-
-
Streptococcus pneumoniae Q07296
-
-
Streptococcus pneumoniae ATCC BAA-334 / TIGR4 Q07296
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration Streptococcus pneumoniae
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
Streptococcus pneumoniae ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
Bacillus anthracis ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
Streptococcus pneumoniae ATCC BAA-334 / TIGR4 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29166, sequence calculation, 2 * 29035, mass spectrometry Streptococcus pneumoniae
dimer 2 * 29701, sequence calculation, 2 * 29569, mass spectrometry Bacillus anthracis
More analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2 Streptococcus pneumoniae
More modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2 Bacillus anthracis

Synonyms

Synonyms Comment Organism
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase
-
Streptococcus pneumoniae
4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase
-
Bacillus anthracis
BaPurC
-
Bacillus anthracis
PurC
-
Streptococcus pneumoniae
PurC
-
Bacillus anthracis
spPurC
-
Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptococcus pneumoniae
25
-
assay at Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Streptococcus pneumoniae
8
-
assay at Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Streptococcus pneumoniae
ATP
-
Bacillus anthracis

General Information

General Information Comment Organism
metabolism 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria Streptococcus pneumoniae
metabolism 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide synthetase (PurC) is a key enzyme in the de novo purine biosynthetic pathway of bacteria Bacillus anthracis
additional information analysis of the dimer structure of enzyme SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview Streptococcus pneumoniae
additional information modeling of the quaternary structure of dimeric enzyme BaPurC, based on the dimer structure of Streptococcus pneumoniae SpPurC, PDB ID 4FE2. Structure comparisons of Streptococcus pneumoniae and Bacillus anthracis PurC enzymes, overview Bacillus anthracis