BRENDA - Enzyme Database
show all sequences of 6.3.2.6

Rapid purification of a bifunctional protein complex possessing phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase

Humble, R.W.; Mackenzie, G.; Shaw, G.; Adv. Exp. Med. Biol. 309B, 223-227 (1991)

Data extracted from this reference:

General Stability
General Stability
Organism
DTT is essential in maintaining the stability, in absence of DTT the activity is completely lost within two hours of extraction
Ovis aries
the ionic strength required to maintain enzyme activity is 0.1 M Tris HCl, at pH 7.8
Ovis aries
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Ovis aries
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
gradient gel electrophoresis of the native protein
Ovis aries
420000
-
gel filtration
Ovis aries
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ovis aries
-
-
-
Purification (Commentary)
Commentary
Organism
copurification of the bifunctional protein complex possessing phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (EC 6.3.2.6)
Ovis aries
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.06
-
-
Ovis aries
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp
-
1266
Ovis aries
ADP + phosphate + 1-(5'-phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
-
-
-
-
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.4
8
narrow stability profile with maximal stability at pH 7.6
Ovis aries
General Stability (protein specific)
General Stability
Organism
DTT is essential in maintaining the stability, in absence of DTT the activity is completely lost within two hours of extraction
Ovis aries
the ionic strength required to maintain enzyme activity is 0.1 M Tris HCl, at pH 7.8
Ovis aries
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Ovis aries
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
96000
-
gradient gel electrophoresis of the native protein
Ovis aries
420000
-
gel filtration
Ovis aries
Purification (Commentary) (protein specific)
Commentary
Organism
copurification of the bifunctional protein complex possessing phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (EC 6.3.2.6)
Ovis aries
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.06
-
-
Ovis aries
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp
-
1266
Ovis aries
ADP + phosphate + 1-(5'-phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
-
-
-
-
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.4
8
narrow stability profile with maximal stability at pH 7.6
Ovis aries
Other publictions for EC 6.3.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746228
Suparmin
Insight into cordycepin biosy ...
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PLoS ONE
12
e0187052
2017
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1
1
1
1
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732397
Manjunath
Catalytic pathway, substrate b ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
J. Struct. Biol.
191
22-31
2015
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1
1
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7
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745942
Goswami
Role and regulation of coordi ...
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23445-23461
2015
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1
2
2
1
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746369
Tuntland
Differences in the purificati ...
Bacillus anthracis, Streptococcus pneumoniae, Streptococcus pneumoniae ATCC BAA-334 / TIGR4
Protein Expr. Purif.
114
143-148
2015
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2
2
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3
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4
2
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2
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4
4
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743939
Wolf
Structures of SAICAR syntheta ...
Streptococcus pneumoniae, Streptococcus pneumoniae ATCC 33400
Acta Crystallogr. Sect. D
70
841-850
2014
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1
1
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1
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3
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3
3
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722404
Manjunath
Structure of SAICAR synthetase ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Int. J. Biol. Macromol.
53
7-19
2013
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1
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5
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1
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1
1
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728030
Manjunath
Molecular dynamics perspective ...
Ehrlichia chaffeensis, Escherichia coli, Geobacillus kaustophilus, Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661, Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428
J. Chem. Inf. Model.
53
2448-2461
2013
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7
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7
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7
5
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5
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3
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3
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7
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7
5
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5
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3
3
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728777
Taschner
Crystal structure of the inver ...
Trichoplusia ni
Proteins
81
1473-1478
2013
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1
1
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2
1
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1
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1
2
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1
1
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701545
Manjunath
Cloning, expression, purificat ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. F
66
180-183
2010
-
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1
1
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2
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-
713651
Cheng
Cloning, expression, purificat ...
Streptococcus suis
Acta Crystallogr. Sect. F
66
909-912
2010
-
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1
1
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1
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4
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1
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1
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703376
Ng
Zebrafish mutations in gart an ...
Danio rerio
Development
136
2601-2611
2009
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1
1
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691703
Zhang
Structural biology of the puri ...
Escherichia coli, Homo sapiens, Saccharomyces cerevisiae, Thermotoga maritima
Cell. Mol. Life Sci.
65
3699-3724
2008
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4
-
4
-
4
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12
4
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4
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4
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4
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12
4
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675427
Li
Octameric structure of the hum ...
Homo sapiens
J. Mol. Biol.
366
1603-1614
2007
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1
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1
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1
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2
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1
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671109
Zhang
Structure of SAICAR synthase f ...
Thermotoga maritima
Acta Crystallogr. Sect. F
62
335-339
2006
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1
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673183
Urusova
-
X-ray diffraction study of the ...
Saccharomyces cerevisiae
Crystallogr. Rep.
51
824-827
2006
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1
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674608
Ginder
Nucleotide complexes of Escher ...
Escherichia coli
J. Biol. Chem.
281
20680-20688
2006
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661204
Nelson
Mechanism of action of Escheri ...
Escherichia coli
Biochemistry
44
766-774
2005
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3
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13
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13
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650908
Urusova
-
X-ray diffraction study of the ...
Saccharomyces cerevisiae
Crystallogr. Rep.
48
763-767
2003
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651425
O'Donnell
The Drosophila melanogaster ad ...
Drosophila melanogaster
Genetics
154
1239-1253
2000
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649890
Meyer
Evidence for the Direct Transf ...
Escherichia coli
Biochemistry
38
3012-3018
1999
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653921
Levdikov
The structure of SAICAR syntha ...
Saccharomyces cerevisiae
Structure
6
363-376
1998
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1269
Senecoff
De novo purine synthesis in Ar ...
Arabidopsis thaliana
Plant Physiol.
112
905-917
1996
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1
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1
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1
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7
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1267
Grebenko
Crystallization and preliminar ...
Saccharomyces cerevisiae
J. Mol. Biol.
228
298-299
1992
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