Cloned (Comment) | Organism |
---|---|
gene pylC, recombinant expression of His-tagged enzyme from operon pylTSBCD in Escherichia coli strain BL21 Tuner (DE3) | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (3R)-3-methyl-D-ornithine + L-lysine | Methanosarcina acetivorans | - |
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | Methanosarcina acetivorans ATCC 35395 | - |
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | Methanosarcina acetivorans DSM 2834 | - |
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | Methanosarcina acetivorans JCM 12185 | - |
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | Q8TUC0 | - |
- |
Methanosarcina acetivorans ATCC 35395 | Q8TUC0 | - |
- |
Methanosarcina acetivorans DSM 2834 | Q8TUC0 | - |
- |
Methanosarcina acetivorans JCM 12185 | Q8TUC0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 Tuner (DE3) by nickel affinity chromatography | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (3R)-3-methyl-D-ornithine + L-lysine | - |
Methanosarcina acetivorans | ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | - |
Methanosarcina acetivorans ATCC 35395 | ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | - |
Methanosarcina acetivorans DSM 2834 | ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
ATP + (3R)-3-methyl-D-ornithine + L-lysine | - |
Methanosarcina acetivorans JCM 12185 | ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-methylornithine-L-lysine ligase | UniProt | Methanosarcina acetivorans |
pylC | - |
Methanosarcina acetivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanosarcina acetivorans |
General Information | Comment | Organism |
---|---|---|
evolution | PylC is a member of the carbamoyl phosphate synthetase family of proteins forming amides between carbon dioxide (or carboxyl groups), and ammonia (or amines) | Methanosarcina acetivorans |
metabolism | proposed pathway of pyrrolysine biosynthesis by the products of pylB, pylC, and pylD from two molecules of lysine. Lysine is first used to make (2R, 3R) 3-methylornithine. After ligation to a second molecule of lysine, the terminal amine of the methylornithyl-Nepsilon-lysine intermediate is oxidized to an aldehyde. Spontaneous elimination of water forms pyrrolysine | Methanosarcina acetivorans |
physiological function | PylC ligates the epsilonN of lysine to the carboxyl of D-ornithine as a first step in forming desmethylpyrrolysine | Methanosarcina acetivorans |