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Literature summary for 6.3.2.59 extracted from

  • Gaston, M.; Zhang, L.; Green-Church, K.; Krzycki, J.
    The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine (2011), Nature, 471, 647-650 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pylC, recombinant expression of His-tagged enzyme from operon pylTSBCD in Escherichia coli strain BL21 Tuner (DE3) Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanosarcina acetivorans
-
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanosarcina acetivorans ATCC 35395
-
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanosarcina acetivorans DSM 2834
-
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanosarcina acetivorans JCM 12185
-
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TUC0
-
-
Methanosarcina acetivorans ATCC 35395 Q8TUC0
-
-
Methanosarcina acetivorans DSM 2834 Q8TUC0
-
-
Methanosarcina acetivorans JCM 12185 Q8TUC0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 Tuner (DE3) by nickel affinity chromatography Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
Methanosarcina acetivorans ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
Methanosarcina acetivorans ATCC 35395 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
Methanosarcina acetivorans DSM 2834 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
Methanosarcina acetivorans JCM 12185 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
?

Synonyms

Synonyms Comment Organism
3-methylornithine-L-lysine ligase UniProt Methanosarcina acetivorans
pylC
-
Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
ATP
-
Methanosarcina acetivorans

General Information

General Information Comment Organism
evolution PylC is a member of the carbamoyl phosphate synthetase family of proteins forming amides between carbon dioxide (or carboxyl groups), and ammonia (or amines) Methanosarcina acetivorans
metabolism proposed pathway of pyrrolysine biosynthesis by the products of pylB, pylC, and pylD from two molecules of lysine. Lysine is first used to make (2R, 3R) 3-methylornithine. After ligation to a second molecule of lysine, the terminal amine of the methylornithyl-Nepsilon-lysine intermediate is oxidized to an aldehyde. Spontaneous elimination of water forms pyrrolysine Methanosarcina acetivorans
physiological function PylC ligates the epsilonN of lysine to the carboxyl of D-ornithine as a first step in forming desmethylpyrrolysine Methanosarcina acetivorans