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Literature summary for 6.3.2.52 extracted from
- The role of JAR1 in jasmonoyl-L-isoleucine production during Arabidopsis wound response (2008), Planta, 227, 1221-1232 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
L-Ile | at pH 8.6 and 25°C | Arabidopsis thaliana |  |
| 0.19 | - |
(-)-jasmonate | at pH 8.6 and 25°C | Arabidopsis thaliana | |
| 1.84 | - |
L-Phe | at pH 8.6 and 25°C | Arabidopsis thaliana | |
| 1.93 | - |
L-Leu | at pH 8.6 and 25°C | Arabidopsis thaliana | |
| 2.49 | - |
L-Val | at pH 8.6 and 25°C | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (-)-jasmonate + L-Ile | Arabidopsis thaliana | L-Ile is the preferred amino acid | AMP + diphosphate + jasmonoyl-L-Ile | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Col-0 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| shoot | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (-)-jasmonate + L-Ile | L-Ile is the preferred amino acid | Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Ile | - |
? | |
| ATP + (-)-jasmonate + L-Leu | - |
Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Leu | - |
? | |
| ATP + (-)-jasmonate + L-Phe | - |
Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Phe | - |
? | |
| ATP + (-)-jasmonate + L-Val | - |
Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Val | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| JAR1 | - |
Arabidopsis thaliana |
| jasmonate: amino acid synthetase | - |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | the enzyme transcript increases dramatically in wounded tissue, reaching a maximum after about 1 h | up |
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