Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + jasmonate + an L-amino acid | Arabidopsis thaliana | - |
AMP + diphosphate + a jasmonoyl-L-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00024 | - |
using (+)-jasmonate as substrate, at pH 8.6 and 25°C | Arabidopsis thaliana |
0.00756 | - |
using 9,10-dihydro-jasmonate as substrate, at pH 8.6 and 25°C | Arabidopsis thaliana |
0.0105 | - |
using (+/-)-jasmonate as substrate, at pH 8.6 and 25°C | Arabidopsis thaliana |
0.0298 | - |
using (-)-jasmonate as substrate, at pH 8.6 and 25°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (+)-jasmonate + L-Ile | very low activity | Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Ile | - |
? | |
ATP + (+/-)-jasmonate + L-Ile | - |
Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Ile | - |
? | |
ATP + (-)-jasmonate + L-Ile | the rate of synthesis of (-)-jasmonate is about 100fold faster than for (+)-jasmonate. Diadenosine tetraphosphate cannot substitute ATP | Arabidopsis thaliana | AMP + diphosphate + jasmonoyl-L-Ile | - |
? | |
ATP + 9,10-dihydro-jasmonate + L-Ile | - |
Arabidopsis thaliana | ? | - |
? | |
ATP + jasmonate + an L-amino acid | - |
Arabidopsis thaliana | AMP + diphosphate + a jasmonoyl-L-amino acid | - |
? | |
additional information | the rate of conjugation with 12-hydroxy-jasmonate and 3-oxo-2-(2Z-pentenyl)cyclopentane-1-butyric acid is about 1-2% that for jasmonate. The enzyme shows no activity with (+/-)-12-oxo-phytodienoic acid, 3-oxo-2-(2Z-pentenyl)cyclopentane-1-hexanoate, and 3-oxo-2-(2Z-pentenyl)cyclopentane-1-octanoate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
JAR1 | - |
Arabidopsis thaliana |
jasmonate:amino acid synthetase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |