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Literature summary for 6.3.2.5 extracted from

  • Yao, J.; Dotson, G.D.
    Kinetic characterization of human phosphopantothenoylcysteine synthetase (2009), Biochim. Biophys. Acta, 1794, 1743-1750.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Homo sapiens
ATP
-
Homo sapiens
CMP
-
Homo sapiens
CTP
-
Homo sapiens
GTP
-
Homo sapiens
UTP
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 Homo sapiens
0.013
-
(R)-4'-phosphopantothenate co-substrate ATP, pH 7.6, 37°C Homo sapiens
0.014
-
L-Cys co-substrate ATP, pH 7.6, 37°C Homo sapiens
0.016
-
L-Cys co-substrate CTP, pH 7.6, 37°C Homo sapiens
0.057
-
(R)-4'-phosphopantothenate co-substrate CTP, pH 7.6, 37°C Homo sapiens
0.265
-
CTP pH 7.6, 37°C Homo sapiens
0.269
-
ATP pH 7.6, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine bi uni uni bi ping pong mechanism. 18O from [carboxyl-18O] phosphopantothenate is incorporated into the AMP or CMP produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate, supporting similar catalytic mechanisms under both CTP and ATP conditions Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-Cys human enzyme can use both ATP and CTP with similar affinity. Catalysis under CTP conditions displays Michaelis-Menten kinetics Homo sapiens (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate
-
?
CTP + (R)-4'-phosphopantothenate + L-Cys human enzyme can use both ATP and CTP with similar affinity. Enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 Homo sapiens (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
CTP pH 7.6, 37°C Homo sapiens
0.56
-
ATP pH 7.6, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.074
-
CTP pH 7.6, 37°C Homo sapiens
0.372
-
GTP pH 7.6, 37°C Homo sapiens
0.465
-
ATP pH 7.6, 37°C Homo sapiens
0.662
-
UTP pH 7.6, 37°C Homo sapiens
3.7
-
AMP pH 7.6, 37°C Homo sapiens
4.69
-
CMP pH 7.6, 37°C Homo sapiens