Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens | |
ATP | - |
Homo sapiens | |
CMP | - |
Homo sapiens | |
CTP | - |
Homo sapiens | |
GTP | - |
Homo sapiens | |
UTP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 | Homo sapiens | |
0.013 | - |
(R)-4'-phosphopantothenate | co-substrate ATP, pH 7.6, 37°C | Homo sapiens | |
0.014 | - |
L-Cys | co-substrate ATP, pH 7.6, 37°C | Homo sapiens | |
0.016 | - |
L-Cys | co-substrate CTP, pH 7.6, 37°C | Homo sapiens | |
0.057 | - |
(R)-4'-phosphopantothenate | co-substrate CTP, pH 7.6, 37°C | Homo sapiens | |
0.265 | - |
CTP | pH 7.6, 37°C | Homo sapiens | |
0.269 | - |
ATP | pH 7.6, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | bi uni uni bi ping pong mechanism. 18O from [carboxyl-18O] phosphopantothenate is incorporated into the AMP or CMP produced during PPCS catalysis, consistent with the formation of a phosphopantothenoyl cytidylate or phosphopantothenoyl adenylate intermediate, supporting similar catalytic mechanisms under both CTP and ATP conditions | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4'-phosphopantothenate + L-Cys | human enzyme can use both ATP and CTP with similar affinity. Catalysis under CTP conditions displays Michaelis-Menten kinetics | Homo sapiens | (R)-4'-phosphopantothenoyl-L-cysteine + ADP + phosphate | - |
? | |
CTP + (R)-4'-phosphopantothenate + L-Cys | human enzyme can use both ATP and CTP with similar affinity. Enzyme shows cooperative binding of ATP, measured as a Hill constant of 1.7 | Homo sapiens | (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
CTP | pH 7.6, 37°C | Homo sapiens | |
0.56 | - |
ATP | pH 7.6, 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
CTP | pH 7.6, 37°C | Homo sapiens | |
0.372 | - |
GTP | pH 7.6, 37°C | Homo sapiens | |
0.465 | - |
ATP | pH 7.6, 37°C | Homo sapiens | |
0.662 | - |
UTP | pH 7.6, 37°C | Homo sapiens | |
3.7 | - |
AMP | pH 7.6, 37°C | Homo sapiens | |
4.69 | - |
CMP | pH 7.6, 37°C | Homo sapiens |