Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.5 extracted from

  • Strauss, E.; Kinsland, C.; Ge, Y.; McLafferty, F.W.; Begley, T.P.
    Phosphopantothenoylcysteine synthetase from Escherichia coli. Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria (2001), J. Biol. Chem., 276, 13513-13516.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
cysteine pH 7.6 Escherichia coli
0.24
-
L-cysteine at pH 7.6 and 25°C Escherichia coli
0.29
-
CTP pH 7.6 Escherichia coli
0.29
-
4'-phosphopantothenate pH 7.6 Escherichia coli
0.29
-
CTP at pH 7.6 and 25°C Escherichia coli
0.29
-
(R)-4'-phosphopantothenate at pH 7.6 and 25°C Escherichia coli
0.78
-
(R)-4'-phosphopantothenoyl-L-cysteine pH 7.6 Escherichia coli
0.78
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine at pH 7.6 and 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine Escherichia coli
-
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
r
CTP + (R)-4'-phosphopantothenate + L-cysteine Escherichia coli
-
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephacryl S-200 column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, HiTrap Blue column chromatography, and Superdex 200 gel filtration Escherichia coli
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.371
-
after 1280fold purification, at pH 7.6 and 25°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
Escherichia coli CTP + (R)-4'-phosphopantothenate + L-cysteine
-
r
CTP + (R)-4'-phosphopantothenate + L-Cys CTP specifically required Escherichia coli (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate
-
r
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
Escherichia coli CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
r
additional information ATP, GTP, and UTP cannot substitute for CTP Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Escherichia coli
? x * 45471, ESI/FTMS Escherichia coli
More enzyme is part of the Dfp flavoprotein Escherichia coli

Synonyms

Synonyms Comment Organism
CoaBC
-
Escherichia coli
Dfp
-
Escherichia coli
phosphopantothenoylcysteine synthetase
-
Escherichia coli
PPC-S
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.83
-
N-[(R)-4'-phosphopantothenoyl]-L-cysteine at pH 7.6 and 25°C Escherichia coli
0.88
-
(R)-4'-phosphopantothenate at pH 7.6 and 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
CTP
-
Escherichia coli
FMN
-
Escherichia coli