Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner(DE3) cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
cysteine | pH 7.6 | Escherichia coli | |
0.24 | - |
L-cysteine | at pH 7.6 and 25°C | Escherichia coli | |
0.29 | - |
CTP | pH 7.6 | Escherichia coli | |
0.29 | - |
4'-phosphopantothenate | pH 7.6 | Escherichia coli | |
0.29 | - |
CTP | at pH 7.6 and 25°C | Escherichia coli | |
0.29 | - |
(R)-4'-phosphopantothenate | at pH 7.6 and 25°C | Escherichia coli | |
0.78 | - |
(R)-4'-phosphopantothenoyl-L-cysteine | pH 7.6 | Escherichia coli | |
0.78 | - |
N-[(R)-4'-phosphopantothenoyl]-L-cysteine | at pH 7.6 and 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | Escherichia coli | - |
CTP + (R)-4'-phosphopantothenate + L-cysteine | - |
r | |
CTP + (R)-4'-phosphopantothenate + L-cysteine | Escherichia coli | - |
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephacryl S-200 column chromatography, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, HiTrap Blue column chromatography, and Superdex 200 gel filtration | Escherichia coli |
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.371 | - |
after 1280fold purification, at pH 7.6 and 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
Escherichia coli | CTP + (R)-4'-phosphopantothenate + L-cysteine | - |
r | |
CTP + (R)-4'-phosphopantothenate + L-Cys | CTP specifically required | Escherichia coli | (R)-4'-phosphopantothenoyl-L-cysteine + CDP + phosphate | - |
r | |
CTP + (R)-4'-phosphopantothenate + L-cysteine | - |
Escherichia coli | CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
r | |
additional information | ATP, GTP, and UTP cannot substitute for CTP | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Escherichia coli |
? | x * 45471, ESI/FTMS | Escherichia coli |
More | enzyme is part of the Dfp flavoprotein | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CoaBC | - |
Escherichia coli |
Dfp | - |
Escherichia coli |
phosphopantothenoylcysteine synthetase | - |
Escherichia coli |
PPC-S | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
N-[(R)-4'-phosphopantothenoyl]-L-cysteine | at pH 7.6 and 25°C | Escherichia coli | |
0.88 | - |
(R)-4'-phosphopantothenate | at pH 7.6 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Escherichia coli | |
FMN | - |
Escherichia coli |