Cloned (Comment) | Organism |
---|---|
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 | Bacillus subtilis |
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 | Bacillus amyloliquefaciens |
gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview | Bacillus subtilis |
gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322 | Bacillus pumilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | several bacilysin-deficient strains, e.g. strain ng79, construction of mutant strains GSB315, GSB319, GSB320, GSB321, and GSB322 | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus pumilus | |
Mg2+ | - |
Bacillus subtilis | |
Mg2+ | - |
Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + L-anticapsin | Bacillus pumilus | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus subtilis | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus amyloliquefaciens | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus subtilis 168 | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus pumilus ATCC 7065 | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus amyloliquefaciens ATCC 15841 | - |
ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | Bacillus subtilis A1/3 | - |
ADP + phosphate + bacilysin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | Q8KWS8 | with plasmid pSB657, gene bacD | - |
Bacillus amyloliquefaciens ATCC 15841 | Q8KWS8 | with plasmid pSB657, gene bacD | - |
Bacillus pumilus | - |
gene bacD | - |
Bacillus pumilus ATCC 7065 | - |
gene bacD | - |
Bacillus subtilis | P39641 | with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE | - |
Bacillus subtilis | Q8KWT3 | with plasmid pSB657, gene bacD | - |
Bacillus subtilis 168 | P39641 | with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE | - |
Bacillus subtilis A1/3 | Q8KWT3 | with plasmid pSB657, gene bacD | - |
no activity in Bacillus amyloliquefaciens | - |
- |
- |
no activity in Bacillus amyloliquefaciens GSB272 | - |
- |
- |
no activity in Bacillus coagulans | - |
- |
- |
no activity in Bacillus licheniformis | - |
- |
- |
no activity in Bacillus licheniformis ATCC 9789 | - |
- |
- |
no activity in Bacillus megaterium | - |
- |
- |
no activity in Bacillus megaterium PV361 | - |
- |
- |
no activity in Bacillus pumilus | - |
- |
- |
no activity in Bacillus pumilus ATCC 12140 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + L-anticapsin | - |
Bacillus pumilus | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus subtilis | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus amyloliquefaciens | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus subtilis 168 | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus pumilus ATCC 7065 | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus amyloliquefaciens ATCC 15841 | ADP + phosphate + bacilysin | - |
? | |
ATP + L-alanine + L-anticapsin | - |
Bacillus subtilis A1/3 | ADP + phosphate + bacilysin | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus pumilus | |
ATP | - |
Bacillus subtilis | |
ATP | - |
Bacillus amyloliquefaciens |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant | Bacillus subtilis |
malfunction | mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant | Bacillus amyloliquefaciens |
additional information | physiological prevention of suicidal antibiotic effects, e.g. for enzyme-deficient strain GSB322 with plasmid pSB674(bacABCD), overview | Bacillus subtilis |
physiological function | the enzyme shows antibiotic activity against various indicator strains as Candida albicans CAP1-312, Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae | Bacillus subtilis |
physiological function | the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae | Bacillus subtilis |
physiological function | the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae | Bacillus amyloliquefaciens |
physiological function | the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae | Bacillus pumilus |