Application | Comment | Organism |
---|---|---|
pharmacology | potential application in industrial protein engineering for the environmentally friendly biological production of useful peptide compounds, such as physiologically active peptides, artificial sweeteners and antibiotics | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme in complex with ADP and an intermediate analog, phosphorylated phosphinate L-alanyl-L-phenylalanine, refined to 2.5 A resolution, sitting-drop vapor-diffusion method at 20°C | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
S1845A | no significant difference in kcat value between the wild type and the mutant enzyme. 2fold increase in Km-value for L-alanine | Bacillus subtilis |
Y75F | no significant difference in kcat value between the wild type and the mutant enzyme, 2fold increase in KM-value for L-phenylalanine | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid | competitive inhibition for L-alanine but not for L-phenylalanine | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-alanine | pH 7.1, 37°C, mutant enzyme Y75F | Bacillus subtilis | |
1.7 | - |
L-alanine | pH 7.7, 37°C, mutant enzyme Y75F | Bacillus subtilis | |
2 | - |
L-alanine | pH 7.7, 37°C, wild-type enzyme | Bacillus subtilis | |
2.6 | - |
L-alanine | pH 7.1, 37°C, wild-type enzyme | Bacillus subtilis | |
4.6 | - |
L-alanine | pH 7.7, 37°C, mutant enzyme S184A | Bacillus subtilis | |
4.9 | - |
L-alanine | pH 7.1, 37°C, mutant enzyme S184F | Bacillus subtilis | |
16 | - |
L-phenylalanine | pH 7.7, 37°C, mutant enzyme S184A | Bacillus subtilis | |
18 | - |
L-phenylalanine | pH 7.7, 37°C, wild-type enzyme | Bacillus subtilis | |
38 | - |
L-phenylalanine | pH 7.1, 37°C, mutant enzyme S184F | Bacillus subtilis | |
44 | - |
L-phenylalanine | pH 7.1, 37°C, wild-type enzyme | Bacillus subtilis | |
45 | - |
L-phenylalanine | pH 7.7, 37°C, mutant enzyme | Bacillus subtilis | |
92 | - |
L-phenylalanine | pH 7.1, 37°C, mutant enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + (1R,2S,6R)-anticapsin | Bacillus subtilis | - |
ADP + phosphate + bacilysin | bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | Bacillus subtilis 168 | - |
ADP + phosphate + bacilysin | bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39641 | - |
- |
Bacillus subtilis 168 | P39641 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP | the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog | Bacillus subtilis | (2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP | - |
? | |
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP | the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog | Bacillus subtilis 168 | (2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP | - |
? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | - |
Bacillus subtilis | ADP + phosphate + bacilysin | bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + (1R,2S,6R)-anticapsin | - |
Bacillus subtilis 168 | ADP + phosphate + bacilysin | bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine | ? | |
ATP + L-alanine + L-phenylalanine | the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity | Bacillus subtilis | ADP + phosphate + L-alanyl-L-phenylalanine | - |
? | |
ATP + L-alanine + L-phenylalanine | the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity | Bacillus subtilis 168 | ADP + phosphate + L-alanyl-L-phenylalanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
BacD | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
L-phenylalanine | pH 7.1, 37°C, mutant enzyme | Bacillus subtilis | |
7.2 | - |
L-alanine | pH 7.1, 37°C, mutant enzyme Y75F | Bacillus subtilis | |
7.7 | - |
L-alanine | pH 7.1, 37°C, wild-type enzyme | Bacillus subtilis | |
7.7 | - |
L-phenylalanine | pH 7.1, 37°C, wild-type enzyme | Bacillus subtilis | |
7.8 | - |
L-alanine | pH 7.1, 37°C, mutant enzyme S184F | Bacillus subtilis | |
7.8 | - |
L-phenylalanine | pH 7.1, 37°C, mutant enzyme S184F | Bacillus subtilis | |
9.7 | - |
L-phenylalanine | pH 7.7, 37°C, mutant enzyme | Bacillus subtilis | |
9.7 | - |
L-alanine | pH 7.7, 37°C, mutant enzyme Y75F | Bacillus subtilis | |
9.7 | - |
L-alanine | pH 7.7, 37°C, wild-type enzyme | Bacillus subtilis | |
9.7 | - |
L-phenylalanine | pH 7.7, 37°C, wild-type enzyme | Bacillus subtilis | |
10.8 | - |
L-alanine | pH 7.7, 37°C, mutant enzyme S184A | Bacillus subtilis | |
10.8 | - |
L-phenylalanine | pH 7.7, 37°C, mutant enzyme S184A | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0154 | - |
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid | pH 7.7, 37°C | Bacillus subtilis |