KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine | pH 8.0, 22°C | Pantoea agglomerans | |
0.072 | - |
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine | pH 8.0, 22°C | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | E2JA31 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine + L-valine | i.e. N-beta-epoxysuccinamoyl-2,3-diaminopropionate. DdaF can catalyze the ligation of both Nbeta-trans-epoxysuccinamoyl-diaminopropionate diastereomers to Val to produce the Nbeta-trans-epoxysuccinamoyl-diaminopropionate-L-valine dipeptide antibiotics. DdaF uses (R,R)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate as a saturable substrate, whereas saturation is not achieved with (S,S)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate at concentrations up to 590 microM | Pantoea agglomerans | ADP + phosphate + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanyl-L-valine | product is a natural antibiotic | ? | |
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine | i.e. Nbeta-fumaramoyl-diaminopropionate | Pantoea agglomerans | ADP + phosphate + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanyl-L-valine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine | pH 8.0, 22°C | Pantoea agglomerans | |
3 | - |
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine | pH 8.0, 22°C | Pantoea agglomerans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.83 | - |
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine | pH 8.0, 22°C | Pantoea agglomerans | |
56.7 | - |
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine | pH 8.0, 22°C | Pantoea agglomerans |