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Literature summary for 6.3.2.47 extracted from

  • Hollenhorst, M.A.; Bumpus, S.B.
    Matthews, M.L.; Bollinger, J.M.Jr.; Kelleher, N.L.; Walsh, C.T.: The nonribosomal peptide synthetase enzyme DdaD tethers N(beta)-fumaramoyl-l-2,3-diaminopropionate for Fe(II)/alpha-ketoglutarate-dependent epoxidation by DdaC during dapdiamide antibiotic biosynthesis (2010), J. Am. Chem. Soc., 132, 15773-15781.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine pH 8.0, 22°C Pantoea agglomerans
0.072
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine pH 8.0, 22°C Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans E2JA31
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine + L-valine i.e. N-beta-epoxysuccinamoyl-2,3-diaminopropionate. DdaF can catalyze the ligation of both Nbeta-trans-epoxysuccinamoyl-diaminopropionate diastereomers to Val to produce the Nbeta-trans-epoxysuccinamoyl-diaminopropionate-L-valine dipeptide antibiotics. DdaF uses (R,R)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate as a saturable substrate, whereas saturation is not achieved with (S,S)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate at concentrations up to 590 microM Pantoea agglomerans ADP + phosphate + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanyl-L-valine product is a natural antibiotic ?
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine i.e. Nbeta-fumaramoyl-diaminopropionate Pantoea agglomerans ADP + phosphate + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanyl-L-valine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine pH 8.0, 22°C Pantoea agglomerans
3
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine pH 8.0, 22°C Pantoea agglomerans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.83
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine pH 8.0, 22°C Pantoea agglomerans
56.7
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine pH 8.0, 22°C Pantoea agglomerans