Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pantoea agglomerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Nbeta-fumaramoyl-diaminopropionate | substrate inhibition above 0.5 mM | Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
L-valine | pH 8.0, 22°C | Pantoea agglomerans | |
0.83 | - |
L-isoleucine | pH 8.0, 22°C | Pantoea agglomerans | |
7.82 | - |
L-leucine | pH 8.0, 22°C | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | E2JA31 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-isoleucine | - |
Pantoea agglomerans | ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-isoleucine | - |
? | |
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-leucine | - |
Pantoea agglomerans | ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-leucine | - |
? | |
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine | - |
Pantoea agglomerans | ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-valine | - |
? | |
additional information | DdaF will not accept Nbeta-fumaroyl-diaminopropionate or Nalpha-fumaramoyl-diaminopropionate. The enzyme requires the fumaroyl moiety to be first converted to the fumaramoyl half amide in Nbeta-fumaramoyl-diaminopropionate | Pantoea agglomerans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DdaF | - |
Pantoea agglomerans |
General Information | Comment | Organism |
---|---|---|
physiological function | gene DdaF belongs to the dapdiamide antibiotic biosynthetic gene cluster. DdaF acts as a second ATP-dependent amide ligase, cleaving ATP to ADP and Pi during amide bond formation. DdaF adds Val, Ile, or Leu to the carboxylate of fumaramoyl-2,3-diaminopropionate to make the three dapdiamides A-C. To build the dapdiamide antibiotic scaffold, amidation must occur on the fumaroyl-diaminopropionate scaffold, after DdaG action but before DdaF catalysis | Pantoea agglomerans |