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Literature summary for 6.3.2.47 extracted from

  • Hollenhorst, M.A.; Clardy, J.; Walsh, C.T.
    The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics (2009), Biochemistry, 48, 10467-10472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea agglomerans

Inhibitors

Inhibitors Comment Organism Structure
Nbeta-fumaramoyl-diaminopropionate substrate inhibition above 0.5 mM Pantoea agglomerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 L-valine pH 8.0, 22°C Pantoea agglomerans
0.83
-
 L-isoleucine pH 8.0, 22°C Pantoea agglomerans
7.82
-
 L-leucine pH 8.0, 22°C Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans E2JA31
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-isoleucine
-
 Pantoea agglomerans ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-isoleucine
-
 ?
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-leucine
-
 Pantoea agglomerans ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-leucine
-
 ?
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine
-
 Pantoea agglomerans ADP + phosphate + 3-(((2E)-4-amino-4-oxobut-2-enoyl)amino)-L-alanyl-L-valine
-
 ?
additional information DdaF will not accept Nbeta-fumaroyl-diaminopropionate or Nalpha-fumaramoyl-diaminopropionate. The enzyme requires the fumaroyl moiety to be first converted to the fumaramoyl half amide in Nbeta-fumaramoyl-diaminopropionate Pantoea agglomerans ?
-
 ?

Synonyms

Synonyms Comment Organism
DdaF
-
 Pantoea agglomerans

General Information

General Information Comment Organism
physiological function gene DdaF belongs to the dapdiamide antibiotic biosynthetic gene cluster. DdaF acts as a second ATP-dependent amide ligase, cleaving ATP to ADP and Pi during amide bond formation. DdaF adds Val, Ile, or Leu to the carboxylate of fumaramoyl-2,3-diaminopropionate to make the three dapdiamides A-C. To build the dapdiamide antibiotic scaffold, amidation must occur on the fumaroyl-diaminopropionate scaffold, after DdaG action but before DdaF catalysis Pantoea agglomerans