Cloned (Comment) | Organism |
---|---|
gene dll, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | homology structure modeling analyses of wild-type enzyme and mutants S293D and S293E | Thermus thermophilus |
S293A | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293C | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293D | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with high production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293E | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with high production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293F | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293G | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293H | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293I | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with high production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293K | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293L | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293M | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with high production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293N | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293P | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme, it catalyzes the synthesis of both D-AlaNH2 and D-Ala-dDAla | Thermus thermophilus |
S293Q | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293R | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme, it catalyzes the synthesis of both D-AlaNH2 and D-Ala-D-Ala | Thermus thermophilus |
S293S | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293T | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293V | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with high production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293W | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
S293Y | site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type enzyme with production of D-AlaNH2 from D-Ala and NH3 in contrast to the wild-type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Thermus thermophilus | |
7.35 | - |
D-alanine | pH 9.0, 40°C, mutant S293E | Thermus thermophilus | |
8.86 | - |
D-alanine | pH 9.0, 40°C, mutant S293D | Thermus thermophilus | |
1530 | - |
NH3 | pH 9.0, 40°C, mutant S293D | Thermus thermophilus | |
1580 | - |
NH3 | pH 9.0, 40°C, mutant S293E | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | Thermus thermophilus | - |
ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
ADP + phosphate + D-alanyl-D-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SHZ3 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SHZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by dialysis | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 D-alanine | - |
Thermus thermophilus | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + 2 D-alanine | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + phosphate + D-alanyl-D-alanine | - |
? | |
ATP + D-Ala + NH3 | the D-AlaNH2 production of enzyme S293X mutants is optimized, the S293E variant, which is selected as the best enzyme for D-AlaNH2 production, exhibits an optimal activity at pH 9.0 and 40°C for D-AlaNH2 production. The S293E variant catalyzes the synthesis of 9.3 and 35.7 mM of D-AlaNH2 from 10 and 50 mM D-Ala and 3 M NH4Cl with conversion yields of 93% and 71.4%, respectively. The S293E variant exhibits higher reaction specificity to D-AlaNH2 production compared with the S293D variant and the other variants | Thermus thermophilus | ADP + phosphate + D-AlaNH2 | - |
? | |
ATP + D-Ala + NH3 | the D-AlaNH2 production of enzyme S293X mutants is optimized, the S293E variant, which is selected as the best enzyme for D-AlaNH2 production, exhibits an optimal activity at pH 9.0 and 40°C for D-AlaNH2 production. The S293E variant catalyzes the synthesis of 9.3 and 35.7 mM of D-AlaNH2 from 10 and 50 mM D-Ala and 3 M NH4Cl with conversion yields of 93% and 71.4%, respectively. The S293E variant exhibits higher reaction specificity to D-AlaNH2 production compared with the S293D variant and the other variants | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + phosphate + D-AlaNH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent D-Ala:D-Ala ligase | - |
Thermus thermophilus |
Ddl | - |
Thermus thermophilus |
TtDdL | - |
Thermus thermophilus |
TTHA1587 | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
D-alanine | pH 9.0, 40°C, mutant S293E | Thermus thermophilus | |
0.092 | - |
D-alanine | pH 9.0, 40°C, mutant S293D | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
additional information | homology structure modeling analyses of wild-type enzyme and mutants S293D and S293E. Both amino acids Arg268 and Ser293 play an important role in the synthesis of D-Ala-D-Ala, residue Ser293 recognizes the carboxylate group of D-Ala2, but is not involved in the ATP hydrolysis, while the Arg268 residue recognizes the carboxylate group of D-Ala1 and is involved in ATP hydrolysis to form the activated acyl-phosphate intermediate. Ligand docking simulations | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
D-alanine | pH 9.0, 40°C, mutant S293E | Thermus thermophilus | |
0.0104 | - |
D-alanine | pH 9.0, 40°C, mutant S293D | Thermus thermophilus |