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Literature summary for 6.3.2.4 extracted from

  • Skedelj, V.; Arsovska, E.; Tomasic, T.; Kroflic, A.; Hodnik, V.; Hrast, M.; Bester-Rogac, M.; Anderluh, G.; Gobec, S.; Bostock, J.; Chopra, I.; O'Neill, A.J.; Randall, C.; Zega, A.
    6-Arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of bacterial D-alanine:D-alanine ligase (2012), PLoS ONE, 7, e39922.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.4

Application

Application Comment Organism
drug development the enzyme is an attractive and viable target in the search for effective antimicrobial drugs Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-(2-amino-6-phenylpyrido[2,3-d]pyrimidin-7-yl)-3-tert-butylurea
-
 Escherichia coli
1-tert-butyl-3-[6-(2,6-dichlorophenyl)-2-[[3-(morpholin-4-yl)propyl]amino]pyrido[2,3-d]pyrimidin-7-yl]urea
-
 Escherichia coli
1-tert-butyl-3-[6-(3,5-dimethoxyphenyl)-2-[[3-(dimethylamino)propyl]amino]pyrido[2,3-d]pyrimidin-7-yl]urea
-
 Escherichia coli
1-tert-butyl-3-[6-(3,5-dimethoxyphenyl)-2-[[3-(morpholin-4-yl)propyl]amino]pyrido[2,3-d]pyrimidin-7-yl]urea
-
 Escherichia coli
1-[2-amino-6-(1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butylurea
-
 Escherichia coli
1-[2-amino-6-(2,6-dibromophenyl)pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butylurea
-
 Escherichia coli
1-[2-amino-6-(2-bromo-6-fluorophenyl)pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butylurea
-
 Escherichia coli
1-[2-amino-6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butylurea
-
 Escherichia coli
1-[2-amino-6-[3,5-bis(trifluoromethyl)phenyl]pyrido[2,3-d]pyrimidin-7-yl]-3-tert-butylurea
-
 Escherichia coli
2-[(1-[[2-amino-6-(2,6-dichlorophenyl)pyrido[2,3-d]pyrimidin-7-yl]amino]ethenyl)amino]propan-2-ol
-
 Escherichia coli
2-[(1-[[2-amino-6-(2,6-difluorophenyl)pyrido[2,3-d]pyrimidin-7-yl]amino]ethenyl)amino]propan-2-ol
-
 Escherichia coli
2-[(1-[[2-amino-6-(3,5-dichlorophenyl)pyrido[2,3-d]pyrimidin-7-yl]amino]ethenyl)amino]propan-2-ol
-
 Escherichia coli
2-[(1-[[2-amino-6-(3,5-difluorophenyl)pyrido[2,3-d]pyrimidin-7-yl]amino]ethenyl)amino]propan-2-ol
-
 Escherichia coli
6-(1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2,6-dibromophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2,6-dichlorophenyl)-N2-[3-(dimethylamino)propyl]pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2,6-dichlorophenyl)-N2-[3-(morpholin-4-yl)propyl]pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2,6-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2,6-difluorophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(2-chloro-6-fluorophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(3,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(3,5-difluorophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(3,5-dimethoxyphenyl)-N2-[3-(morpholin-4-yl)propyl]pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-(3,5-dimethoxyphenyl)pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-phenylpyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
6-[3,5-bis(trifluoromethyl)phenyl]pyrido[2,3-d]pyrimidine-2,7-diamine
-
 Escherichia coli
additional information 6-arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of the bacterial enzyme, ligand docking and modeling, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2 D-alanine Escherichia coli
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine reaction mechanism via tetrahedral reaction intermediate, overview Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2 D-alanine
-
 Escherichia coli ADP + phosphate + D-alanyl-D-alanine
-
 ?

Synonyms

Synonyms Comment Organism
D-Alanine:D-alanine ligase
-
 Escherichia coli
Ddl
-
 Escherichia coli
DdlB
-
 Escherichia coli
DdlB ligase
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction inhibition of Ddl prevents bacterial growth Escherichia coli
physiological function the enzyme catalyzes the formation of the terminal D-Ala-D-Ala dipeptide of the peptidoglycan precursor UDP-MurNAc pentapeptide Escherichia coli