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Literature summary for 6.3.2.31 extracted from

  • Nocek, B.; Evdokimova, E.; Proudfoot, M.; Kudritska, M.; Grochowski, L.L.; White, R.H.; Savchenko, A.; Yakunin, A.F.; Edwards, A.; Joachimiak, A.
    Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus - a member of a new family of non-ribosomal peptide synthases (2007), J. Mol. Biol., 372, 456-469.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.31

Cloned(Commentary)

Cloned (Comment) Organism
-
 Archaeoglobus fulgidus DSM 4304

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme from Archaeoglobus fulgidus and its complex with GDP at 2.5 A and 1.35 A resolution, respectively. CofE-AF crystallization is performed by the sitting-drop and hanging-drop methods using vapor diffusion at 18°C Archaeoglobus fulgidus DSM 4304

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ there are two Mn2+-binding sites per monomer within close proximity of the GDP alpha and beta-phosphate groups Archaeoglobus fulgidus DSM 4304

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + GTP + coenzyme F420-0 Archaeoglobus fulgidus DSM 4304 the enzyme protein catalyzes two distinct and independent reactions, firstly attaching a glutamte via its alpha-NH2 to F420-0. The second reaction (cf. coenzyme F420-1:gamma-glutamyl ligase) is a gamma ligation, taking place when a certain amount of monoglutamylated F420-1 has accumulated GDP + phosphate + coenzyme gamma-F420-1
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus DSM 4304 O28028
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus DSM 4304

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + GTP + coenzyme F420-0 the enzyme protein catalyzes two distinct and independent reactions, firstly attaching a glutamte via its alpha-NH2 to F420-0. The second reaction (cf. coenzyme F420-1:gamma-glutamyl ligase) is a gamma ligation, taking place when a certain amount of monoglutamylated F420-1 has accumulated Archaeoglobus fulgidus DSM 4304 GDP + phosphate + coenzyme gamma-F420-1
-
 ?

Synonyms

Synonyms Comment Organism
CofE-AF
-
 Archaeoglobus fulgidus DSM 4304

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Archaeoglobus fulgidus DSM 4304

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Archaeoglobus fulgidus DSM 4304