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Literature summary for 6.3.2.30 extracted from

  • Krehenbrink, M.; Steinbüchel, A.
    Partial purification and characterization of a non-cyanobacterial cyanophycin synthetase from Acinetobacter calcoaceticus strain ADP1 with regard to substrate specificity, substrate affinity and binding to cyanophycin (2004), Microbiology, 150, 2599-2608.
    View publication on PubMed
Search for more literature for 6.3.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH1 cells Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 ATP 30°C, pH 8.2, for the L-aspartic acid-ATP binding site alone Acinetobacter calcoaceticus
0.24
-
 L-aspartic acid 30°C, pH 8.2 Acinetobacter calcoaceticus
0.278
-
 ATP 30°C, pH 8.2, for the complete reaction Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Acinetobacter calcoaceticus
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
additional information Acinetobacter calcoaceticus without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, gel filtration, affinity precipitation with cyanophycin, 69fold purification Acinetobacter calcoaceticus

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre Acinetobacter calcoaceticus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.265
-
 30°C, pH 8.2 Acinetobacter calcoaceticus

Storage Stability

Storage Stability Organism
at 7°C the purified enzyme is stable for about one week, thereafter the activity decreases rapidly, storage at -20°C in the presence of 10% (w/v) DMSO or 50% (w/v) glycerol does not improve the stability significantly Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Acinetobacter calcoaceticus ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
L-aspartic acid + ATP
-
 Acinetobacter calcoaceticus poly-L-aspartic acid + ADP + phosphate
-
 ?
additional information without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis Acinetobacter calcoaceticus ?
-
 ?

Synonyms

Synonyms Comment Organism
cyanophycin synthetase
-
 Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 30°C Acinetobacter calcoaceticus