Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TOP10 cells | Synechocystis sp. PCC 6803 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ATP | 28°C, pH 8.2 | Synechocystis sp. PCC 6803 | |
0.45 | - |
L-aspartic acid | 28°C, pH 8.2 | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Synechocystis sp. PCC 6803 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
2 * 90000, SDS-PAGE | Synechocystis sp. PCC 6803 |
240000 | - |
gel filtration | Synechocystis sp. PCC 6803 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | P73833 | - |
- |
Purification (Comment) | Organism |
---|---|
from Synechocystis and from recombinant Escherichia coli cells | Synechocystis sp. PCC 6803 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre | Synechocystis sp. PCC 6803 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.238 | - |
after 72fold purification, 28°C, pH 8.2 | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | normal assay conditions | Synechocystis sp. PCC 6803 | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
L-aspartic acid + ATP | - |
Synechocystis sp. PCC 6803 | poly-L-aspartic acid + ADP + phosphate | - |
? | |
additional information | no activity without L-aspartic acid, L-glutamic acid cannot substitute for L-aspartic acid | Synechocystis sp. PCC 6803 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 90000, SDS-PAGE | Synechocystis sp. PCC 6803 |
Synonyms | Comment | Organism |
---|---|---|
CphA | - |
Synechocystis sp. PCC 6803 |
cyanophycin synthetase | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme becomes inactive after 30 min incubation at this temperature | Synechocystis sp. PCC 6803 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
still active after 30 min incubation | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
Tris buffer | Synechocystis sp. PCC 6803 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9.7 | - |
Synechocystis sp. PCC 6803 |