Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.30 extracted from

  • Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A.
    Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity (2001), Appl. Environ. Microbiol., 67, 2176-2182.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TOP10 cells Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
ATP 28°C, pH 8.2 Synechocystis sp. PCC 6803
0.45
-
L-aspartic acid 28°C, pH 8.2 Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
2 * 90000, SDS-PAGE Synechocystis sp. PCC 6803
240000
-
gel filtration Synechocystis sp. PCC 6803

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P73833
-
-

Purification (Commentary)

Purification (Comment) Organism
from Synechocystis and from recombinant Escherichia coli cells Synechocystis sp. PCC 6803

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre Synechocystis sp. PCC 6803

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.238
-
after 72fold purification, 28°C, pH 8.2 Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg normal assay conditions Synechocystis sp. PCC 6803 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
?
L-aspartic acid + ATP
-
Synechocystis sp. PCC 6803 poly-L-aspartic acid + ADP + phosphate
-
?
additional information no activity without L-aspartic acid, L-glutamic acid cannot substitute for L-aspartic acid Synechocystis sp. PCC 6803 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 90000, SDS-PAGE Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
CphA
-
Synechocystis sp. PCC 6803
cyanophycin synthetase
-
Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
enzyme becomes inactive after 30 min incubation at this temperature Synechocystis sp. PCC 6803

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
28
-
still active after 30 min incubation Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
Tris buffer Synechocystis sp. PCC 6803

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9.7
-
Synechocystis sp. PCC 6803