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Literature summary for 6.3.2.3 extracted from

  • Slavens, K.D.; Brown, T.R.; Barakat, K.A.; Cundari, T.R.; Anderson, M.E.
    Valine 44 and valine 45 of human glutathione synthetase are key for subunit stability and negative cooperativity (2011), Biochem. Biophys. Res. Commun., 410, 597-601.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.3

Protein Variants

Protein Variants Comment Organism
V44A decrease in melting temperature, slight decrease in activity Homo sapiens
V44A/V45A decrease in melting temperature, initial activity similar to wild-type Homo sapiens
V44W decrease in melting temperature, 16% decrease in activity Homo sapiens
V45A decrease in melting temperature, slight decrease in activity Homo sapiens
V45W decrease in melting temperature, 30% decrease in activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 gamma-L-Glu-aminobutanoate mutant V45W, pH 8.2, 37°C Homo sapiens
0.95
-
 gamma-L-Glu-aminobutanoate mutant V44W, pH 8.2, 37°C Homo sapiens
1.42
-
 gamma-L-Glu-aminobutanoate mutant V44A/V45A, pH 8.2, 37°C Homo sapiens
1.42
-
 gamma-L-Glu-aminobutanoate wild-type, pH 8.2, 37°C Homo sapiens
1.54
-
 gamma-L-Glu-aminobutanoate mutant V45A, pH 8.2, 37°C Homo sapiens
1.6
-
 gamma-L-Glu-aminobutanoate mutant V44A, pH 8.2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-L-Glu-aminobutanoate + Gly
-
 Homo sapiens ADP + phosphate + ophthalmic acid
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41.3
-
 melting temperature, mutant V45W Homo sapiens
42.8
-
 melting temperature, mutant V44A/V45A Homo sapiens
47.8
-
 melting temperature, mutant V45A Homo sapiens
51.1
-
 melting temperature, mutant V44A Homo sapiens
57
-
 melting temperature, mutant V44W Homo sapiens
60.4
-
 melting temperature, wild-type Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.6
-
 gamma-L-Glu-aminobutanoate mutant V45W, pH 8.2, 37°C Homo sapiens
16.4
-
 gamma-L-Glu-aminobutanoate mutant V44W, pH 8.2, 37°C Homo sapiens
17.4
-
 gamma-L-Glu-aminobutanoate mutant V45A, pH 8.2, 37°C Homo sapiens
18.9
-
 gamma-L-Glu-aminobutanoate mutant V44A, pH 8.2, 37°C Homo sapiens
19.5
-
 gamma-L-Glu-aminobutanoate wild-type, pH 8.2, 37°C Homo sapiens
21.7
-
 gamma-L-Glu-aminobutanoate mutant V44A/V45A, pH 8.2, 37°C Homo sapiens