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Literature summary for 6.3.2.3 extracted from
- Glutathione synthesis in Streptococcus agalactiae. One protein accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities (2005), J. Biol. Chem., 280, 11829-11839.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptococcus agalactiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.9 | - |
gamma-L-Glu-L-Cys | - |
Streptococcus agalactiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities, gel filtration | Streptococcus agalactiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus agalactiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His6-gamma-GCS-GS, bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.33 | - |
- |
Streptococcus agalactiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, -20°C or -80°C, the isolated enzyme loses activity quickly (about 50% in 24 h), with or without glycerol | Streptococcus agalactiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-Glu-L-Cys + Gly | - |
Streptococcus agalactiae | ADP + phosphate + glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-GCS-GS | bifunctional enzyme accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities | Streptococcus agalactiae |
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Release 2023.1 (January 2023)
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