Cloned (Comment) | Organism |
---|---|
expression in Schizosaccharomyces pombe, results in 1.4fold higher glutathione content and 1.9fold ncreased enzyme activity, regulation by the Atf1-Spc-1-Wis1 signal pathway | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | wild-type cells are not able to grow on cadmium chloride containing substrate, but the recombinant strain is able to survive at 1 mM cadmium chloride concentration, the wild-type can grow on 1 mM Cd2+ in presence of 20 mM GSH | Schizosaccharomyces pombe | |
Hg2+ | wild-type cells are not able to grow on mercuric chloride containing substrate, but the recombinant strain is able to survive at 0.01 mM mercuric chloride concentration, the wild-type can grow on 0.01 mM Hg2+ in presence of 20 mM GSH | Schizosaccharomyces pombe | |
L-buthionine-(S,R)-sulfoximine | induction of enzyme expression at 0.01 mM | Schizosaccharomyces pombe | |
menadione | wild-type cells are not able to grow on menadione containing substrate, but the recombinant strain is able to survive at 0.1 mM menadione, induction of enzyme expression by superoxide generation | Schizosaccharomyces pombe | |
additional information | no induction of enzyme expression by thioacetamide | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-Cys + Gly | Schizosaccharomyces pombe | gene is regulated by stresses via the Atf1-Spc-1-Wis1 signal pathway | ADP + phosphate + glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + gamma-L-Glu-L-Cys + Gly | - |
Schizosaccharomyces pombe | ADP + phosphate + glutathione | - |
? | |
ATP + gamma-L-Glu-L-Cys + Gly | gene is regulated by stresses via the Atf1-Spc-1-Wis1 signal pathway | Schizosaccharomyces pombe | ADP + phosphate + glutathione | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glutathione synthetase | - |
Schizosaccharomyces pombe |
GS | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Schizosaccharomyces pombe |