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Literature summary for 6.3.2.3 extracted from
- A spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells (2002), Chem. Biol. Interact., 140, 49-65.
General Stability
| General Stability | Organism |
|---|---|
| glycerol is required for stabilization of the enzyme during freezing | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by acivicin | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-Glu-L-Cys + Gly | Rattus norvegicus | - |
ADP + phosphate + glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from kidney to homogeneity, partially from liver | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| M22 cell | tumorigenic cell line derived from OC22 by reiterated treatment with N-methyl-N-nitro-N-nitrosoguanidine | Rattus norvegicus | - |
| OC/CDE22 cell | a non-tumorigenic cell line of oval cells established from livers of rats undergoing carcinogenesis by the choline deficient/ethionine-supplemented diet for 12 weeks | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay development, activity under different reaction conditions in rat liver | Rattus norvegicus |
| 0.0039 | - |
cell line OC/CDE22, GSH depleted | Rattus norvegicus |
| 0.0047 | - |
cell line M22 | Rattus norvegicus |
| 0.0064 | - |
cell line OC/CDE22 | Rattus norvegicus |
| 0.0078 | - |
cell line M22, GSH depleted | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, 30% glycerol, at least 2 years stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-Glu-L-Cys + Gly | - |
Rattus norvegicus | ADP + phosphate + glutathione | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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