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Literature summary for 6.3.2.3 extracted from
- Cooperative binding of gamma-glutamyl substrate to human glutathione synthetase (2001), Biochem. Biophys. Res. Commun., 289, 80-84.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of cooperative substrate binding | Homo sapiens | |
| 0.063 | - |
gamma-glutamyl-alpha-aminobutyrate | pH 8.2, 37°C | Homo sapiens |  |
| 0.248 | - |
ATP | pH 8.2, 37°C | Homo sapiens | |
| 0.452 | - |
glycine | pH 8.2, 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-L-Glu-L-Cys + Gly | Homo sapiens | final step of glutathione biosynthesis | ADP + phosphate + glutathione | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli, to homogeneity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + gamma-glutamyl-alpha-aminobutyrate | - |
Homo sapiens | ? | - |
? | |
| ATP + gamma-L-Glu-L-Cys + Gly | gamma-glutamyl-cysteine is bound cooperatively by the enzyme, ATP might play a key role in sequential binding, but its amount does not alter the cooperative binding of gamma-glutamyl-cysteine | Homo sapiens | ADP + phosphate + glutathione | - |
? | |
| ATP + gamma-L-Glu-L-Cys + Gly | final step of glutathione biosynthesis | Homo sapiens | ADP + phosphate + glutathione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glutathione synthetase | - |
Homo sapiens |
| GS | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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