General Stability | Organism |
---|---|
loss of activity by exposure to Mn2+ for long periods | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | inhibition of ADP-ATP exchange reaction, no effect on tripeptide synthesis | Rattus norvegicus | |
NEM | inhibition of ADP-ATP exchange reaction, no effect on tripeptide synthesis | Rattus norvegicus | |
PCMB | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
ATP | - |
Rattus norvegicus | |
0.76 | - |
Gly | - |
Rattus norvegicus | |
25 | - |
N-acetyl-L-Cys | - |
Rattus norvegicus | |
60 | - |
N-acetyl-L-2-aminobutyrate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 77% of the efficiency relative to Mg2+ | Rattus norvegicus | |
K+ | no requirement for exchange reaction | Rattus norvegicus | |
K+ | strict requirement for monovalent cation, maximal activity with K+ | Rattus norvegicus | |
Li+ | can replace K+ | Rattus norvegicus | |
Mg2+ | divalent metal ion required, Mg2+ is most effective | Rattus norvegicus | |
Mn2+ | 73% of the efficiency relative to Mg2+ | Rattus norvegicus | |
NH4+ | can replace K+ | Rattus norvegicus | |
Rb+ | can replace K+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE | Rattus norvegicus |
118000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains about 2% carbohydrate | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
stable for more than 2 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-Cys + Gly | - |
Rattus norvegicus | ADP + phosphate + acetyl-Cys-Gly | - |
? | |
ATP + beta-aminoglutaryl-L-2-aminobutyrate + Gly | 15% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + beta-aminoglutaryl-L-2-aminobutyryl-Gly | - |
? | |
ATP + DL-gamma-(beta-methyl)Glu-L-2-aminobutyrate + Gly | 44% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + DL-gamma-(beta-methyl)Glu-L-2-aminobutyryl-Gly | - |
? | |
ATP + DL-gamma-(gamma-methyl)Glu-L-2-aminobutyrate + Gly | 16% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + DL-gamma-(gamma-methyl)Glu-L-2-aminobutyryl-Gly | - |
? | |
ATP + gamma-Glu-aminobutyrate + Gly | L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + ophthalmic acid | - |
? | |
ATP + gamma-Glu-aminobutyrate + Gly | D-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + ophthalmic acid | - |
? | |
ATP + gamma-Glu-L-Cys + aminooxyacetate | 53% of the activity relative to Gly | Rattus norvegicus | ADP + phosphate + gamma-Glu-Cys-aminoxyacetate | - |
? | |
ATP + gamma-Glu-L-Cys + Gly | - |
Rattus norvegicus | ADP + phosphate + glutathione | - |
? | |
ATP + gamma-Glu-L-Cys + N-hydroxyglycine | 33% of the activity relative to Gly | Rattus norvegicus | ADP + phosphate + gamma-Glu-Cys-N-hydroxyglycine | - |
? | |
ATP + gamma-Glu-S-methylcysteine + Gly | 24% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + gamma-Glu-S-methylcysteinyl-Gly | - |
? | |
ATP + Glu-L-2-aminobutyrate + Gly | 16% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + gamma-Glu-L-Cys-Gly | - |
? | |
ATP + L-gamma-(alpha-methyl)Glu-L-2-aminobutyrate + Gly | - |
Rattus norvegicus | ADP + phosphate + L-gamma-(alpha-methyl)Glu-L-2-aminobutyryl-Gly | - |
? | |
ATP + L-gamma-(N-methyl)Glu-L-2-aminobutyrate + Gly | 52% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + L-gamma-(N-methyl)Glu-L-2-aminobutyryl-Gly | - |
? | |
ATP + L-gamma-Glu-Gly + Gly | 8% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + L-gamma-Glu-Gly-Gly | - |
? | |
ATP + L-gamma-Glu-L-2-aminopentanoic acid + Gly | 9% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + gamma-Glu-S-methylcysteinyl-Gly | - |
? | |
ATP + L-gamma-Glu-L-Ala + Gly | 55% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + L-gamma-Glu-L-Ala-Gly | - |
? | |
ATP + L-gamma-Glu-L-Cys + Gly | 102% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + L-gamma-Glu-L-Cys-Gly | - |
? | |
ATP + L-gamma-Glu-L-Ser + Gly | 77% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + L-gamma-Glu-L-Ser-Gly | - |
? | |
ATP + N-acetyl-L-2-aminobutyrate + Gly | 8% of the activity relative to L-gamma-glutamyl-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + N-acetyl-L-2-aminobutyrate-Gly | - |
? | |
ATP + N-acetyl-L-Cys + Gly | 20% of the activity relative to L-gamma-Glu-L-2-aminobutyrate | Rattus norvegicus | ADP + phosphate + N-acetyl-L-Cys-Gly | - |
? | |
CTP + gamma-Glu-L-Cys + Gly | 4% of the activity relative to ATP | Rattus norvegicus | CDP + phosphate + glutathione | - |
? | |
dATP + gamma-Glu-L-Cys + Gly | 75% of the activity relative to ATP | Rattus norvegicus | dADP + phosphate + glutathione | - |
? | |
GTP + gamma-Glu-L-Cys + Gly | 3% of the activity relative to ATP | Rattus norvegicus | GDP + phosphate + glutathione | - |
? | |
ITP + gamma-Glu-L-Cys + Gly | 6% of the activity relative to ATP | Rattus norvegicus | IDP + phosphate + glutathione | - |
? | |
additional information | the enzyme catalyzes ADP-ATP exchange at 0.62% of the rate of tripeptide synthesis | Rattus norvegicus | ? | - |
? | |
UTP + gamma-Glu-L-Cys + Gly | 24% of the activity relative to ATP | Rattus norvegicus | UDP + phosphate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
5 min, stable in absence of glutathione or other thiols | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.7 | about 50% of maximal activity at pH 7.0 and 9.7 | Rattus norvegicus |