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Literature summary for 6.3.2.3 extracted from

  • Dennda, G.; Kula, M.R.
    Purification and evaluation of the glutathione-synthesizing enzymes from Candida boidinii for cell-free synthesis of glutathione (1986), J. Biotechnol., 4, 143-158.
No PubMed abstract available
Search for more literature for 6.3.2.3

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, strong [Candida] boidinii
CN-
-
 [Candida] boidinii
Fe2+ 2 mM, strong [Candida] boidinii
GSSG no inhibition by reduced glutathione [Candida] boidinii
iodoacetate
-
 [Candida] boidinii
NEM
-
 [Candida] boidinii
PCMB
-
 [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 gamma-Glu-Cys
-
 [Candida] boidinii
0.48
-
 ATP
-
 [Candida] boidinii
0.8
-
 Gly
-
 [Candida] boidinii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 5fold stimulation at 40 mM [Candida] boidinii
Mg2+ activates [Candida] boidinii
Mn2+ 40% of the activation rate of Mg2+, in the optimal range: 2-4 mM [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 2 * 59000, SDS-PAGE [Candida] boidinii
120000
-
 gel filtration [Candida] boidinii

Organism

Organism UniProt Comment Textmining
Brettanomyces abstinens
-
-
-
Cyberlindnera jadinii
-
-
-
Ogataea angusta
-
-
-
Pichia kudriavzevii
-
-
-
Pichia membranifaciens
-
-
-
Saccharomyces cerevisiae
-
-
-
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
-
 [Candida] boidinii

Storage Stability

Storage Stability Organism
-18°C, stable for at least 1 year [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
 Cyberlindnera jadinii ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Saccharomyces cerevisiae ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Ogataea angusta ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Pichia membranifaciens ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Brettanomyces abstinens ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Pichia kudriavzevii ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 [Candida] boidinii ADP + phosphate + glutathione i.e. gamma-Glu-Cys-Gly ?

Subunits

Subunits Comment Organism
dimer 2 * 59000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 8.3, 40% loss of activity after 1 h in 0.15 M Tris-HCl buffer [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 [Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 maximal stability after 24 h [Candida] boidinii