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Literature summary for 6.3.2.3 extracted from

  • Kato, H.; Chihara, M.; Nishioka, T.; Murata, K.; Kimura, A.; Oda, J.
    Homology of Escherichia coli B glutathione synthetase with dihydrofolate reductase in amino acid sequence and substrate binding site (1987), J. Biochem., 101, 207-215.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
the gene for glutathione synthetase of Escherichia coli B is cloned onto vector plasmid pBR325. Escherichia coli C600 cells transformed by a recombinant plasmid pBR325 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
7,8-dihydrofolate
-
 Escherichia coli
ATP substrate inhibition above 15 mM Escherichia coli
methotrexate
-
 Escherichia coli
NADPH weak Escherichia coli
trimethoprim
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.85
-
 ATP
-
 Escherichia coli
3.33
-
 gamma-Glu-Cys
-
 Escherichia coli
3.53
-
 Gly
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 B
-
Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.83
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli ADP + phosphate + glutathione
-
 ?
ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + glutathione
-
 ?