synthesis |
expression of the cyanophycin synthetase of Synechocystis sp. PCC 6308 in Pseudomonas putida ATCC 4359 using an optimised medium for cultivation, results in synthesis of insoluble cyanophycin up to 14.7 w/w and soluble cyanophycin amounting up to 28.7 w/w of the cell dry matter. The soluble CGP is composed of 50.4 mol% aspartic acid, 32.7 mol% arginine, 8.7 mol% citrulline and 8.3 mol% lysine. The insoluble cyanophycin contains less than 1 mol% of citrulline. Using a mineral salt medium with 1.25 or 2% w/v sodium succinate, respectively, plus 23.7 mM L-arginine, the cells synthesise insoluble cyanophycin amounting up to 25% to 29% of the CDM with only a very low citrulline content |
Synechocystis sp. |