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Literature summary for 6.3.2.29 extracted from

  • Wiefel, L.; Broeker, A.; Steinbuechel, A.
    Synthesis of a citrulline-rich cyanophycin by use of Pseudomonas putida ATCC 4359 (2011), Appl. Microbiol. Biotechnol., 90, 1755-1762.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression of the cyanophycin synthetase of Synechocystis sp. PCC 6308 in Pseudomonas putida ATCC 4359 using an optimised medium for cultivation, results in synthesis of insoluble cyanophycin up to 14.7 w/w and soluble cyanophycin amounting up to 28.7 w/w of the cell dry matter. The soluble CGP is composed of 50.4 mol% aspartic acid, 32.7 mol% arginine, 8.7 mol% citrulline and 8.3 mol% lysine. The insoluble cyanophycin contains less than 1 mol% of citrulline. Using a mineral salt medium with 1.25 or 2% w/v sodium succinate, respectively, plus 23.7 mM L-arginine, the cells synthesise insoluble cyanophycin amounting up to 25% to 29% of the CDM with only a very low citrulline content Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pseudomonas putida ATCC 4359 Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P56947 cf. EC 6.3.2.30
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Synechocystis sp. PCC 6308 P56947 cf. EC 6.3.2.30
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