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Literature summary for 6.3.2.29 extracted from

  • Steinle, A.; Oppermann-Sanio, F.B.; Reichelt, R.; Steinbuechel, A.
    Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae (2008), Appl. Environ. Microbiol., 74, 3410-3418.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n + L-Asp Synechocystis sp.
-
ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC 6308, gene cphA6308
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
Synechocystis sp. ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
?

Synonyms

Synonyms Comment Organism
CphA
-
Synechocystis sp.
cyanophycin synthetase
-
Synechocystis sp.
More cf. EC 6.3.2.30 Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
Synechocystis sp.