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Literature summary for 6.3.2.29 extracted from

  • Aboulmagd, E.; Oppermann-Sanio, F.B.; Steinbüchel, A.
    Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308 (2000), Arch. Microbiol., 174, 297-306.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TOP10 cells Geminocystis herdmanii PCC 6308

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-
Synechocystis sp. PCC 6804
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from Synechocystis and from recombinant Escherichia coli cells Geminocystis herdmanii PCC 6308

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n + L-Asp = ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp i.e. the second reaction of cyanophycin synthesis catalysed by cyanophycin synthase, the first reaction is catalysed by the enzyme's other active centre Geminocystis herdmanii PCC 6308

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0091
-
enzyme isolated from Synechocystis cells, 28°C Geminocystis herdmanii PCC 6308
0.027
-
recombinant enzyme isolated from Escherichia coli Geminocystis herdmanii PCC 6308

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n + L-Asp [L-Asp(4-L-Arg)]n-Asp is a cyanophycin molecule with a C-terminal L-Asp residue that is not linked to an L-Arg residue via its beta-carboxy group, this intermediate is produced in the first reaction catalysed by cyanophycin synthase Geminocystis herdmanii PCC 6308 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp no formation of AMP ?

Synonyms

Synonyms Comment Organism
CphA
-
Geminocystis herdmanii PCC 6308
cyanophycin synthetase
-
Geminocystis herdmanii PCC 6308